Protocol

Microbial Carotenoids From Fungi

Volume 898 of the series Methods in Molecular Biology pp 275-283

Date:

Production of Torularhodin, Torulene, and β-Carotene by Rhodotorula Yeasts

  • Martín MolinéAffiliated withLaboratorio de Microbiología Aplicada y Biotecnología Bariloche, INIBIOMA (CONICET-Universidad del COMAHUE) Email author 
  • , Diego LibkindAffiliated withLaboratorio de Microbiología Aplicada y Biotecnología Bariloche, INIBIOMA (CONICET-Universidad del COMAHUE)
  • , María van BroockAffiliated withLaboratorio de Microbiología Aplicada y Biotecnología Bariloche, INIBIOMA (CONICET-Universidad del COMAHUE)

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Yeasts of the genera Rhodotorula are able to synthesize different pigments of high economic value like β-carotene, torulene, and torularhodin, and therefore represent a biotechnologically interesting group of yeasts. However, the low production rate of pigment in these microorganisms limits its industrial application. Here we describe some strategies to obtain hyperpigmented mutants of Rhodotorula mucilaginosa by means of ultraviolet-B radiation, the procedures for total carotenoids extraction and quantification, and a method for identification of each pigment.

Key words

Torularhodin β-Carotene Carotenoids Rhodotorula Mutants UV-B radiation