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Microbial Carotenoids From Fungi pp 171–182Cite as

Production, Extraction, and Quantification of Astaxanthin by Xanthophyllomyces dendrorhous or Haematococcus pluvialis: Standardized Techniques

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 898))

Abstract

For many years, benefits and disadvantages of pigments production either by microalgae or yeasts have been under analysis. In this contribution we shall deal with Xanthophyllomyces dendrorhous (formerly Phaffia rhodozyma) and Haematococcus pluvialis, which are known as major prominent microorganisms able to synthesize astaxanthin pigment. Then, the usual trend is to look for optimal conditions to conduct astaxanthin synthesis. From one side, pigment production by H. pluvialis is promoted under cellular stress conditions like nutrient deprivation, exposition to high light intensity, aeration. On the other side, X. dendrorhous is able to show significant increase in astaxanthin synthesis when grown in natural carbon sources like coconut milk, grape juice. The main aim of this chapter is to describe optimal environmental conditions for astaxanthin production by X. dendrorhous or H. pluvialis.

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Acknowledgments

The author expresses her gratitude to TESE for the experimental facilities and to CINVESTAV for giving me a non-salary work permit along the development of this research work. I would also like to thank Dr. Jorge A. Torres-Muñoz for the fruitful discussions along the preparation of the manuscript.

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Authors and Affiliations

  1. Department of Biotechnology and Bioengineering, CINVESTAV, Mexico, DF, Mexico

    Alma Rosa Domínguez-Bocanegra

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  1. Alma Rosa Domínguez-Bocanegra
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Correspondence to Alma Rosa Domínguez-Bocanegra .

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  1. Parque Tecnológico de León, Gadea Biopharma, Boecillo 47151, Valladolid, 24009, León, Spain

    José-Luis Barredo

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Domínguez-Bocanegra, A.R. (2012). Production, Extraction, and Quantification of Astaxanthin by Xanthophyllomyces dendrorhous or Haematococcus pluvialis: Standardized Techniques. In: Barredo, JL. (eds) Microbial Carotenoids From Fungi. Methods in Molecular Biology, vol 898. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-918-1_11

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  • DOI: https://doi.org/10.1007/978-1-61779-918-1_11

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-917-4

  • Online ISBN: 978-1-61779-918-1

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