Abstract
The Monterey Bay Aquarium Research Institute’s Environmental Sample Processor (ESP) is well established as an innovative sampling and instrument platform for sensors designed for in situ monitoring of microorganisms in the ocean. The platform can be deployed for periods up to 3 months and real-time data can be remotely downloaded at any time. A daughter platform, the microfluidic block (MFB), is used as an interface between the ESP and analytical modules to permit rapid deployment of new sensor technologies. We have developed a capillary waveguide biosensor (CWB) which employs nucleic acid hybridization for detection and quantification of specific microorganisms, and have integrated it with the MFB for use with the ESP. An important aspect of the CWB is the use of a combined capillary waveguide/hybridization surface that can be regenerated for repeated use. This chapter describes design issues related to the integration of the CWB and MFB and the development of coating protocols to maximize the operational life of the capillary.
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Acknowledgments
We appreciate the funding support from NSF through the EAGER award OCE0929238. We also express our thanks to Dr. Eli Hatchwell for giving us access to microarray equipment at the Stony Brook Medical Center’s Genomic Core Facility and for many fruitful discussions of hybridization kinetics. Discussions on silanes with Qingzhi Zhu of the School of Marine and Atmospheric Sciences were instrumental in our choice of attachment chemistry. This work certainly would not have been possible without the support of Chris Scholin and his staff at MBARI. Harban S. Dhadwal thanks his graduate students William Kaunds, Jason Yen, and Manish Muttreja for working tirelessly on the different components of hardware and software.
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Dhadwal, H.S., Radway, J., Aller, J., Kemp, P., Clements, J. (2012). Development of a Capillary Waveguide Biosensor Analytical Module for Use with the MBARI Environmental Sample Processor. In: Tiquia-Arashiro, S. (eds) Molecular Biological Technologies for Ocean Sensing. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-915-0_2
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