Skip to main content
SpringerLink
Log in
Book cover

Kidney Development pp 23–30Cite as

In Vitro Induction of Nephrogenesis in Mouse Metanephric Mesenchyme with Lithium Introduction and Ureteric Bud Recombination

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 886))

Abstract

The organ culture setup of embryonic kidney has served as a model of nephrogenesis for several decades. In vitro culture of the mouse metanephric mesenchyme enables easy manipulation and analysis of the tissue and provides information of cellular interactions, morphogenesis, cell differentiation, and molecular biology of the developmental process. The advantages of the tissue culture method include enhanced representativeness of situation in living organism compared to cell culture assays and less demanding and time-consuming possibilities to experimental work compared with in vivo research.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Saxén L (1987) Organogenesis of the kidney. Cambridge University Press, Cambridge, UK

    Book  Google Scholar 

  2. Gilbert SF (2000) Paraxial and intermediate mesoderm. In: Gilbert SF (ed) Developmental biology, 6th edn. Sinauer Associated, Inc, Sunderland, MA, p 470

    Google Scholar 

  3. Carroll TJ, Park JS, Hayashi S, Majumdar A, McMahon AP (2005) Wnt9b plays a central role in the regulation of mesenchymal to epithelial transitions underlying organogenesis of the mammalian urogenital system. Dev Cell 9:283–293

    Article  CAS  PubMed  Google Scholar 

  4. Stark K, Vainio S, Vassileva G, McMahon AP (1994) Epithelial transformation of metanephric mesenchyme in the developing kidney regulated by Wnt-4. Nature 372:679–683

    Article  CAS  PubMed  Google Scholar 

  5. Kispert A, Vainio S, McMahon AP (1998) Wnt-4 is a mesenchymal signal for epithelial transformation of metanephric mesenchyme in the developing kidney. Development 125:4225–4234

    CAS  PubMed  Google Scholar 

  6. Grobstein B (1955) Inductive interaction in the development of the mouse metanephros. J Exp Zool 130:319–340

    Article  Google Scholar 

  7. Hedgepeth CM, Conrad LJ, Zhang J et al (1997) Activation of Wnt signalling pathway: a molecular mechanism for lithium activation. Dev Biol 185:82–91

    Article  CAS  PubMed  Google Scholar 

  8. Davies JA, Garrod DR (1995) Induction of early stages of kidney tubule differentiation by lithium ions. Dev Biol 167:50–60

    Article  CAS  PubMed  Google Scholar 

  9. Lin Y, Liu A, Zhang S et al (2001) Induction of ureter bud branching as a response to Wnt-2b signalling during early kidney organogenesis. Dev Dyn 222:26–39

    Article  CAS  PubMed  Google Scholar 

  10. Lin Y, Zhang S, Tuukkanen J et al (2003) Patterning parameters associated with the branching of the ureteric bud regulated by epithelial-mesenchymal interactions. Int J Dev Biol 47:3–13

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Institute of Biomedicine, University of Oulu, Oulu, Finland

    Kimmo Halt

  2. Department of Medical Biochemistry and Molecular Biology, Institute of Biomedicine, Faculty of Medicine, University of Oulu, Oulu, Finland

    Seppo Vainio

Authors
  1. Kimmo Halt
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seppo Vainio
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kimmo Halt .

Editor information

Editors and Affiliations

  1. Hammer Health Sciences Center Department of Genetics and Development, Columbia University Medical Center, New York, NY, USA

    Odyssé Michos

Rights and permissions

Reprints and permissions

Copyright information

© 2012 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Halt, K., Vainio, S. (2012). In Vitro Induction of Nephrogenesis in Mouse Metanephric Mesenchyme with Lithium Introduction and Ureteric Bud Recombination. In: Michos, O. (eds) Kidney Development. Methods in Molecular Biology™, vol 886. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-851-1_3

Download citation

  • DOI: https://doi.org/10.1007/978-1-61779-851-1_3

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-850-4

  • Online ISBN: 978-1-61779-851-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation