Abstract
We describe a simple and inexpensive plant micropropagation system for giant reed (Arundo donax L.) that uses axillary buds from the lateral stems of elite plants selected from field- or nursery-grown plants. The buds, attached to the stems are cultured in stationary liquid MS culture medium, supplemented with indole 3-acetic acid and kinetin. This formulation is the only one required for all the stages. Contrary to what happens in semisolid medium where roots are not formed, the plants cultured in liquid medium are whole plants with shoots and roots that develop at the same time. The survival rate of these plants when transferred to soil is close to 100% during acclimatization. A clonal line of 900 plants from a single mother plant can be produced in 4 months.
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Acknowledgments
Special thanks to Luis Ender Mena-Kú and Isaías Xool-García for their technical support.
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Herrera-Alamillo, M.Á., Robert, M.L. (2012). Liquid In Vitro Culture for the Propagation of Arundo donax . In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 877. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-818-4_12
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DOI: https://doi.org/10.1007/978-1-61779-818-4_12
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