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Somatic Stem Cells pp 89–107Cite as

Identification, Isolation, and Culture of Intestinal Epithelial Stem Cells from Murine Intestine

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 879))

Abstract

The study of adult stem cell populations provides insight into the mechanisms that regulate tissue maintenance in normal physiology and many disease states. With an impressive rate of epithelial renewal driven by a pool of multipotent stem cells, the intestine is a particularly advantageous model system for the study of adult stem cells. Until recently, the isolation and in vitro study of intestinal epithelial stem cells (IESCs) was not possible due to the lack of biomarkers and culture techniques. However, advances in molecular characterization and culture of IESCs have made in vitro studies on this cell type amenable to most laboratories. The methods described in this chapter will allow the investigator to adapt newly established techniques toward downstream analysis of IESCs in vitro.

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Acknowledgments

We would like to thank Victoria Bali, Ph.D., for critical reading of the document. Additionally, we would like to acknowledge Jill Carrington, Ph.D., who to our knowledge was the first to coin the term “cryptoid.” The original work described in this chapter was funded by the National Institutes of Health, 1-K01-DK080181-01, the American Gastroenterological Association Research Scholar Award, the North Carolina Biotechnology Center Grant, and the UNC-Chapel Hill the Center for Gastrointestinal Biology and Disease, 5P30DK034987 (S.T. Magness).

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Authors and Affiliations

  1. Department of Medicine, Division of Gastroenterology and Hepatology and Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

    A. D. Gracz

  2. Departments of Medicine and Biomedical Engineering, Division of Gastroenterology and Hepatology, University of North Carolina and North Carolina State University, Chapel Hill, NC, USA

    B. J. Puthoff

  3. Departments of Medicine and Biomedical Engineering, Division of Gastroenterology, and Hepatology, University of North Carolina and North Carolina State University, Chapel Hill, NC, USA

    S. T. Magness

Authors
  1. A. D. Gracz
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  2. B. J. Puthoff
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  3. S. T. Magness
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Corresponding author

Correspondence to S. T. Magness .

Editor information

Editors and Affiliations

  1. Mouse Cancer Genetics Program, National Cancer Institute, Center Drive 31, Frederick, 21702, Maryland, USA

    Shree Ram Singh

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Gracz, A.D., Puthoff, B.J., Magness, S.T. (2012). Identification, Isolation, and Culture of Intestinal Epithelial Stem Cells from Murine Intestine. In: Singh, S. (eds) Somatic Stem Cells. Methods in Molecular Biology, vol 879. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-815-3_6

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  • DOI: https://doi.org/10.1007/978-1-61779-815-3_6

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-814-6

  • Online ISBN: 978-1-61779-815-3

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