Abstract
Proliferation in mouse oesophageal epithelial cells is confined to the basal layer of the epithelium. Within this population, it is possible to discriminate different sub-populations using a combination of cell kinetic studies and functional assays. In particular, it is possible to distinguish basal epithelial cells, which are post-mitotic and destined to leave the basal layer and differentiate compared with those cells that remain in the cycling pool. Within the cycling basal population, there appears to be a hierarchy with respect to the rate of cell turnover which may reflect a hierarchy of “stemness”, although it has not been possible to demonstrate functional differences between these populations using current in vivo tissue reconstitution assays. The aim of this chapter is to describe the development of a quantitative in vivo tissue reconstitution assay to assess the potency of candidate stem cell populations within the mouse oesophageal epithelium.
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References
Lajtha LG (1979) Stem cell concepts. Differentiation 14:23–34
Bradford GB, Williams B, Rossi R, Bertoncello I (1997) Quiescence, cycling, and turnover in the primitive hematopoietic stem cell compartment. Exp Hematol 25:445–453
Cotsarelis G, Sun TT, Lavker RM (1990) Label-retaining cells reside in the bulge area of pilosebaceous unit: implications for follicular stem cells, hair cycle, and skin carcinogenesis. Cell 61:1329–1337
Croagh D, Phillips W, Redvers R, Thomas R, Kaur P (2007) Identification of candidate murine stem esophageal stem cells using a combination of cell kinetic studies and cell surface markers. Stem Cell 25:313–318
Li A, Pouliot N, Redvers R, Kaur P (2004) Extensive tissue-regenerative capacity of neonatal human keratinocyte stem cells and their progeny. J Clin Invest 113: 390–400
Redvers RP, Kaur P (2005) Serial cultivation of primary adult murine keratinocytes. Methods Mol Biol 289:15–22
Takagi N, Sugimoto M, Yamaguchi S, Ito M, Tan SS, Okabe M (2002) Nonrandom X chromosome inactivation in mouse embryos carrying Searle’s T(X;16)16H translocation visualized using X-linked LACZ and GFP transgenes. Cytogenet Genome Res 99:52–58
Acknowledgements
This work was supported, in part, by a Surgeon-Scientist Fellowship from the Royal Australasian College of Surgeons and a postgraduate research scholarship from the National Health and Medical Research Council of Australia (NHMRC) to D.C. and a NHMRC project grant to W.P. and P.K.
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Croagh, D., Redvers, R., Phillips, W.A., Kaur, P. (2012). Developing a Quantitative In Vivo Tissue Reconstitution Assay to Assess the Relative Potency of Candidate Populations of Mouse Oesophageal Epithelial Cells. In: Singh, S. (eds) Somatic Stem Cells. Methods in Molecular Biology, vol 879. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-815-3_5
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DOI: https://doi.org/10.1007/978-1-61779-815-3_5
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Publisher Name: Humana Press, Totowa, NJ
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