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Generation and Staining of Intestinal Stem Cell Lineage in Adult Midgut

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Somatic Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 879))

Abstract

Stem cell-mediated tissue repair is a promising approach in regenerative medicine. Intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. Recently, using lineage tracing and molecular marker labeling, intestinal stem cells (ISCs) have been identified in Drosophila adult midgut. ISCs reside at the basement membrane and are multipotent as they produce both enterocytes and enteroendocrine cells. The adult Drosophila midgut provides an excellent in vivo model organ to study ISC behavior during aging, stress, regeneration, and infection. It has been demonstrated that Notch, Janus kinase/signal transducer and activator of transcription, epidermal growth factor receptor/mitogen-activated protein kinase, Hippo, and wingless signaling pathways regulate ISCs proliferation and differentiation. There are plenty of genetic tools and markers developed in recent years in Drosophila stem cell studies. These tools and markers are essential in the precise identification of stem cells as well as manipulation of genes in stem cell regulation. Here, we describe the details of genetic tools, markers, and immunolabeling techniques used in identification and characterization of adult midgut stem cells in Drosophila.

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Acknowledgments

M.K.S. is supported by the Knight’s Templar Eye Foundation and start-up support from the University of Dayton, OH. This research was supported by the Intramural Research Program of the National Institutes of Health, National Cancer Institute.

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Authors and Affiliations

  1. Mouse Cancer Genetics Program, National Cancer Institute, Frederick, MD, USA

    Shree Ram Singh & Steven X. Hou

  2. Department of Math and Science, Alabama State University, Montgomery, AL, USA

    Manoj K. Mishra

  3. Department of Biology, University of Dayton, Dayton, OH, USA

    Madhuri Kango-Singh

Authors
  1. Shree Ram Singh
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  2. Manoj K. Mishra
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  3. Madhuri Kango-Singh
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  4. Steven X. Hou
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Corresponding author

Correspondence to Shree Ram Singh .

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Editors and Affiliations

  1. Mouse Cancer Genetics Program, National Cancer Institute, Center Drive 31, Frederick, 21702, Maryland, USA

    Shree Ram Singh

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Singh, S.R., Mishra, M.K., Kango-Singh, M., Hou, S.X. (2012). Generation and Staining of Intestinal Stem Cell Lineage in Adult Midgut. In: Singh, S. (eds) Somatic Stem Cells. Methods in Molecular Biology, vol 879. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-815-3_4

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  • DOI: https://doi.org/10.1007/978-1-61779-815-3_4

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-814-6

  • Online ISBN: 978-1-61779-815-3

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