Abstract
Human embryonic stem cells (hESCs) are an invaluable cell source to study human embryogenesis and development and for exploring the nature of human diseases. Moreover, hESCs can serve as an unlimited source of cells for cell therapy. The first hESC lines were derived from frozen blastocyst-stage embryos. In the past 12 years, the field evolved and the hESC lines are derived from pre-embryos in various developmental stages using several techniques. In parallel, the wide use of hESCs triggered the development of materials and methods for expansion of the cell lines derived. Here, we describe our method for derivation, expansion, and characterization of hESC lines from pre-embryos that were diagnosed to carry aneuploid cells and were destined to be discarded.
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Acknowledgment
The above research was performed in Dr. Nissim Benvenisty’s laboratory at the Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA and we thank him for the privilege of working under his supervision. We thank Kavita Narwani and Berhan Mandefro for excellent technical assistance. This work was partially supported by a CIRM grant # RL1-00636-1.
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Biancotti, JC., Lavon, N. (2012). Derivation, Expansion, and Characterization of Human Embryonic Stem Cell Lines from Aneuploid Embryos. In: Turksen, K. (eds) Human Embryonic Stem Cells Handbook. Methods in Molecular Biology, vol 873. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-794-1_10
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DOI: https://doi.org/10.1007/978-1-61779-794-1_10
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Publisher Name: Humana Press, Totowa, NJ
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Online ISBN: 978-1-61779-794-1
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