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Animal Models of Acute Neurological Injuries II pp 107–114Cite as

Blood Flow Reduction: Laser Doppler, 14C-IAP

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Abstract

Ischemic strokes are caused by a sharp fall of the cerebral blood flow (CBF) to certain regions of brain tissues. In experimental research of cerebral ischemia, the animal models are produced by a variety of methods to reduce or stop the blood supply to the brain, such as using suture, embolus, or direct cut of artery. The reduction in CBF is the key for making ischemic models; therefore, the monitoring of regional CBF is an important experimentation in the field of stroke research. This chapter introduces the common methods for monitoring CBF, with focus on the laser Doppler flowmetry, Carbon-14 iodoantipyrine autoradiography, and laser speckle flowmetry.

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Author information

Authors and Affiliations

  1. Department of Neurology and the Center for Cerebrovascular Disease Research, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    Feng Zhang, Steven H. Graham & Jun Chen

Authors
  1. Feng Zhang
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  2. Steven H. Graham
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  3. Jun Chen
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Corresponding author

Correspondence to Jun Chen .

Editor information

Editors and Affiliations

  1. School of Medicine, University of Pittsburgh, Terrace St. 3500, Pittsburgh, 15213, Pennsylvania, USA

    Jun Chen

  2. School of Medicine, Stark Neurosciences Res. Inst., Indiana University, W. Walnut St. 950, Indianapolis, 46202, Indiana, USA

    Xiao-Ming Xu

  3. School of Medicine, Department of Anatomy & Cell Biology, Indiana University, Barnhill Dr. 635, Indianapolis, 46202, Indiana, USA

    Zao C. Xu

  4. School of Medicine, Dept. Neurosurgery, Loma Linda University, Anderson St. 11234, Loma Linda, 92354, California, USA

    John H. Zhang

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Zhang, F., Graham, S.H., Chen, J. (2012). Blood Flow Reduction: Laser Doppler, 14C-IAP. In: Chen, J., Xu, XM., Xu, Z., Zhang, J. (eds) Animal Models of Acute Neurological Injuries II. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-61779-782-8_11

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  • DOI: https://doi.org/10.1007/978-1-61779-782-8_11

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-781-1

  • Online ISBN: 978-1-61779-782-8

  • eBook Packages: Springer Protocols

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