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Animal Models of Acute Neurological Injuries II pp 99–105Cite as

Morphological Assessments of Focal Cerebral Ischemia: White Matter Injury

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Abstract

A common type of ischemic stroke, focal cerebral ischemia causes nonselective tissue damage in the ischemic area, including damage to both gray and white matter. White matter is made up of neuronal axons and their surrounding oligodendrocytic myelin sheath, and is susceptible to ischemic injury. The contribution of white matter injury to neurological dysfunction was largely ignored in the past, but its significance has been increasingly noticed in recent years. This chapter introduces the classic methods for studying white matter injury after brain ischemia, including immunohistochemical staining of amyloid precursor protein and myelin basic protein. Tissue preparation, staining procedures, evaluation methods, and alternative methods are discussed.

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Author information

Authors and Affiliations

  1. Department of Neurology and the Center for Cerebrovascular Disease Research, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    Feng Zhang

  2. Toronto Western Research Institute, University Health Network, Toronto, ON, Canada

    Lyanne C. Schlichter

  3. Department of Physiology, University of Toronto, Toronto, ON, Canada

    Lyanne C. Schlichter

Authors
  1. Feng Zhang
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  2. Lyanne C. Schlichter
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Corresponding author

Correspondence to Feng Zhang .

Editor information

Editors and Affiliations

  1. School of Medicine, University of Pittsburgh, Terrace St. 3500, Pittsburgh, 15213, Pennsylvania, USA

    Jun Chen

  2. School of Medicine, Stark Neurosciences Res. Inst., Indiana University, W. Walnut St. 950, Indianapolis, 46202, Indiana, USA

    Xiao-Ming Xu

  3. School of Medicine, Department of Anatomy & Cell Biology, Indiana University, Barnhill Dr. 635, Indianapolis, 46202, Indiana, USA

    Zao C. Xu

  4. School of Medicine, Dept. Neurosurgery, Loma Linda University, Anderson St. 11234, Loma Linda, 92354, California, USA

    John H. Zhang

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Zhang, F., Schlichter, L.C. (2012). Morphological Assessments of Focal Cerebral Ischemia: White Matter Injury. In: Chen, J., Xu, XM., Xu, Z., Zhang, J. (eds) Animal Models of Acute Neurological Injuries II. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-61779-782-8_10

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  • DOI: https://doi.org/10.1007/978-1-61779-782-8_10

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-781-1

  • Online ISBN: 978-1-61779-782-8

  • eBook Packages: Springer Protocols

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