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Analysis of Protein Posttranslational Modifications Using DIGE-Based Proteomics

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Difference Gel Electrophoresis (DIGE)

Part of the book series: Methods in Molecular Biology ((MIMB,volume 854))

Abstract

Difference gel electrophoresis (DIGE) is most often used to assess relative changes in the expression levels of individual proteins in multiple complex samples, and this information is valuable in making inferences about relative protein activity. However, a protein’s activity is not solely dependent upon its expression level. A change in activity may also be influenced by myriad posttranslational modifications (PTMs), including palmitoylation, ubiquitination, oxidation, and phosphorylation. In this chapter, we describe the use of DIGE to determine specific PTMs by introducing specific labels or changes in pI and/or molecular weight.

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Acknowledgments

This work was supported by the UNC Systems-Proteomics Center and the Duke Neuroproteomics Center.

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Authors and Affiliations

  1. Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, NC, USA

    Robert M. DeKroon, Joseph S. Y. Jeong, Eric Hamlett & Oscar Alzate

  2. UNC Systems-Proteomics Center, Program of Molecular Biology and Biotechnology, School of Medicine, University of North Carolina, Chapel Hill, NC, USA

    Jennifer B. Robinette, Cristina Osorio & Mihaela Mocanu

Authors
  1. Robert M. DeKroon
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  2. Jennifer B. Robinette
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  3. Cristina Osorio
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  4. Joseph S. Y. Jeong
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  5. Eric Hamlett
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  6. Mihaela Mocanu
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  7. Oscar Alzate
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Corresponding author

Correspondence to Oscar Alzate .

Editor information

Editors and Affiliations

  1. BioCentre, University of Reading, Reading, United Kingdom

    Rainer Cramer

  2. SERVA Electrophoresis GmbH, Carl-Benz-Strasse 7, Heidelberg, 69115, Germany

    Reiner Westermeier

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© 2012 Springer Science+Business Media, LLC

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DeKroon, R.M. et al. (2012). Analysis of Protein Posttranslational Modifications Using DIGE-Based Proteomics. In: Cramer, R., Westermeier, R. (eds) Difference Gel Electrophoresis (DIGE). Methods in Molecular Biology, vol 854. Humana Press. https://doi.org/10.1007/978-1-61779-573-2_9

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  • DOI: https://doi.org/10.1007/978-1-61779-573-2_9

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-572-5

  • Online ISBN: 978-1-61779-573-2

  • eBook Packages: Springer Protocols

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