Abstract
The diagnostic benefits of array comparative genomic hybridisation (CGH) have been demonstrated, with this technique now being applied as the first-line test for patients with intellectual disabilities and/or multiple congenital anomalies in numerous laboratories. There are no technical barriers preventing the introduction of array CGH to prenatal diagnosis. The question is rather how this is best implemented, and for whom. The challenges lie in the interpretation of copy number variations, particularly those which exhibit reduced penetrance or variable expression, and how to deal with incidental findings, which are not related to the observed foetal anomalies, or unclassified variants which are currently of uncertain clinical significance. Recently, applications of array technologies to the field of pre-implantation genetic diagnosis have also been demonstrated. It is important to address the ethical questions raised concerning the genome-wide analysis of prenatal samples to ensure the maximum benefit for patients. We provide an overview of the recent developments on the use of array CGH in the prenatal setting, and address the challenges posed.
Key words
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- cffDNA:
-
Cell-free foetal DNA
- UCV:
-
Unclassified variants
- WGA:
-
Whole-genome amplification
- CNV:
-
Copy number variation
- POC:
-
Product of conception
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Acknowledgements
Paul Brady is funded by a Marie-Curie Early Stage Research Fellowship (MEST-CT-2005-019707). Part of the foetal therapy programme is funded by the European Commission in the 6th framework programme (LSHC-CT-2006-037409) and the Flemish Regional Government (IWT/070715). JDP is a clinical researcher for the Fonds voor Wetenschappelijk Onderzoek Vlaanderen (1.8.012.07.N.02). KD is a senior clinical investigator of the F.W.O. Flanders. Part of this work was made possible by Grants from the IWT (SBO-60848), FWO (GOA/2006/12), and Center of Excellence SymBioSys (Research Council K.U.Leuven EF/05/007), JRV.
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Brady, P.D., Devriendt, K., Deprest, J., Vermeesch, J.R. (2012). Array-Based Approaches in Prenatal Diagnosis. In: Feuk, L. (eds) Genomic Structural Variants. Methods in Molecular Biology, vol 838. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-507-7_7
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