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Proteoglycans pp 131–144Cite as

Glycosaminoglycans: Oligosaccharide Analysis by Liquid Chromatography, Capillary Electrophoresis, and Specific Labeling

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 836))

Abstract

Glycosaminoglycans (GAGs) are a class of biopolymers that include chondrotin sulfate, dermatan sulfate, keratan sulfate, hyaluronic acid, heparin, and heparan sulfate. The GAGs are linear polysaccharides that are microheterogeneous in composition and polydisperse in size. Because they have the most complex structures, this article is aimed at describing a step-by-step procedure for processing and analyzing heparin and heparan sulfate-derived oligosaccharides, although the basic protocols and procedures apply equally well to other members of the GAG family. The methods described in this manuscript include the preparation of oligosaccharides through enzymatic depolymerization, size fractionation by preparative scale size-exclusion chromatography (SEC), and disaccharide isomer analysis by reverse-phase ion-pair high-performance liquid chromatography (RPIP-HPLC) and capillary electrophoresis (CE).

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Acknowledgments

The authors gratefully acknowledge financial support from the National Science Foundation grant CHE 0848976. Sz. B. gratefully acknowledges support from KTIA-OTKA MB08A/80066.

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Authors and Affiliations

  1. Department of Chemistry, University of California—Riverside, Riverside, CA, USA

    Derek J. Langeslay, Christopher J. Jones, Szabolcs Beni & Cynthia K. Larive

  2. Department of Pharmaceutical Chemistry, Semmelweis University, Budapest, Hungary

    Szabolcs Beni

Authors
  1. Derek J. Langeslay
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  2. Christopher J. Jones
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  3. Szabolcs Beni
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  4. Cynthia K. Larive
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Correspondence to Cynthia K. Larive .

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  1. , Faculté de Médicine, INSERM U957 - EA 3822, rue Gaston Veil 1, Nantes, 44035, France

    Françoise Rédini

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Langeslay, D.J., Jones, C.J., Beni, S., Larive, C.K. (2012). Glycosaminoglycans: Oligosaccharide Analysis by Liquid Chromatography, Capillary Electrophoresis, and Specific Labeling. In: Rédini, F. (eds) Proteoglycans. Methods in Molecular Biology, vol 836. Humana Press. https://doi.org/10.1007/978-1-61779-498-8_9

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  • DOI: https://doi.org/10.1007/978-1-61779-498-8_9

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