Protocol

Liver Stem Cells

Volume 826 of the series Methods in Molecular Biology pp 103-114

Date:

Generation and Hepatic Differentiation of Human iPS Cells

  • Tetsuya IshikawaAffiliated withCore Facilities for Research and Innovative Medicine, National cancer Center Research Institute Email author 
  • , Keitaro HagiwaraAffiliated withDivision of Molecular and Cellular Medicine, National Cancer Center Research Institute
  • , Takahiro OchiyaAffiliated withDivision of Molecular and Cellular Medicine, National Cancer Center Research Institute

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Abstract

A method for the generation of human induced pluripotent stem (iPS) cells was established. This method employs adenovirus carrying the ecotropic retrovirus receptor mCAT1 and Moloney murine leukemia virus (MMLV)-based retroviral vectors carrying the four transcription factors POU5F1 (OCT3/4), KLF4, SOX2, and MYC (c-Myc) (Masaki H & Ishikawa T Stem Cell Res 1:105–15, 2007). The differentiation of human iPS cells into hepatic cells was performed by a stepwise protocol (Song Z et al. Cell Res 19:1233–42, 2009). These cells have potential as patient-specific in vitro models for studying disease etiology and could be used in drug discovery programs tailored to deal with genetic variations in drug efficacy and toxicity.

Key words

Human iPS cells POU5F1 (OCT3/4) KLF4 Sox2 MYC (c-Myc) Retrovirus Hepatic differentiation