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DNA Methylation Analysis of Germ Cells by Using Bisulfite-Based Sequencing Methods

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Book cover Germline Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 825))

Abstract

Dynamic changes in DNA methylation at the gene-specific and genome-wide level occur during mammalian germ-cell development. However, the details of how the methylation profiles change remain largely unknown. Bisulfite sequencing analysis is a powerful technique to determine the methylation status of DNA at individual cytosine-guanine dinucleotide (CpG) sites and requires only a small amount of DNA for analysis. Here, we introduce two methods for bisulfite-based DNA methylation analyses using small samples such as germ cells: bisulfite Sanger sequencing at a specific locus and high-throughput bisulfite sequencing at the whole genome level.

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Authors and Affiliations

  1. Department of BioScience, Tokyo University of Agriculture, Tokyo, Japan

    Hisato Kobayashi & Tomohiro Kono

Authors
  1. Hisato Kobayashi
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  2. Tomohiro Kono
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Correspondence to Tomohiro Kono .

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Editors and Affiliations

  1. , School of Biomedical Sciences, The Chinese University of Hong Kong, n/a, Shatin, New Territories, n/a, Hong Kong/PR China

    Wai-Yee Chan

  2. , Animal Biosciences, ARS-USDA, Baltimore Avenue 10300, Beltsville, 20705, Maryland, USA

    Le Ann Blomberg

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Kobayashi, H., Kono, T. (2012). DNA Methylation Analysis of Germ Cells by Using Bisulfite-Based Sequencing Methods. In: Chan, WY., Blomberg, L. (eds) Germline Development. Methods in Molecular Biology, vol 825. Springer, New York, NY. https://doi.org/10.1007/978-1-61779-436-0_17

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  • DOI: https://doi.org/10.1007/978-1-61779-436-0_17

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-61779-435-3

  • Online ISBN: 978-1-61779-436-0

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