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Primary Human Osteoblast Cultures

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Book cover Bone Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 816))

Abstract

Osteoblast cultures can be used to investigate the mechanisms of bone formation, to probe the cellular and molecular basis of bone disease, and to screen for potential therapeutic agents that affect bone formation. Here, we describe the methods for establishing and characterising primary human osteoblast cultures.

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Author information

Authors and Affiliations

  1. Bone and Joint Research Group, Musculoskeletal Biology, Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool, UK

    Jane P. Dillon, Peter J. M. Wilson & James A. Gallagher

  2. Royal Veterinary College, London, UK

    Victoria J. Waring-Green

  3. Lancaster Medical School, Faculty of Health and Medicine, University of Lancaster, Lancaster, UK

    Adam M. Taylor

  4. Musculoskeletal Research Group, Institute of Cellular Medicine, The Medical School, Newcastle University, Newcastle-upon-Tyne, UK

    Mark Birch

  5. Academic Unit of Bone Biology, Department of Human Metabolism, Mellanby Centre for Bone Research, University of Sheffield, Sheffield, UK

    Alison Gartland

Authors
  1. Jane P. Dillon
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  2. Victoria J. Waring-Green
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  3. Adam M. Taylor
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  4. Peter J. M. Wilson
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  5. Mark Birch
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  6. Alison Gartland
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  7. James A. Gallagher
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Corresponding author

Correspondence to James A. Gallagher .

Editor information

Editors and Affiliations

  1. Institute of Medical Sciences, University of Aberdeen, Forester Hill, Aberdeen, AB25 2ZD, United Kingdom

    Miep H. Helfrich

  2. Institute of Genetics and Molecular Medi, Rheumatic Diseases Unit, University of Edinburgh, Crewe Road South, Edinburgh, EH4 2XU, United Kingdom

    Stuart H. Ralston

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© 2012 Springer Science+Business Media, LLC

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Dillon, J.P. et al. (2012). Primary Human Osteoblast Cultures. In: Helfrich, M., Ralston, S. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 816. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-415-5_1

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  • DOI: https://doi.org/10.1007/978-1-61779-415-5_1

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-414-8

  • Online ISBN: 978-1-61779-415-5

  • eBook Packages: Springer Protocols

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