Abstract
Micro and nanoscale protein patterning based on microcontact printing technique on large substrates have often resolution problems due to roof collapse of the poly(dimethylsiloxane) (PDMS) stamps used. Here, we describe a technique that overcomes these issues by using instead a stamp made of poly(methyl methacrylate) (PMMA), a much more rigid polymer that do not collapse even using stamps with very high aspect ratios (up to 300:1). Conformal contact between the stamp and the substrate is achieved because of the homogeneous pressure applied via the nanoimprint lithography instrument, and it has allowed us to print lines of protein 150 nm wide, at a 400 nm period. This technique, therefore, provides an excellent method for the direct printing of high-density submicrometer scale patterns, or, alternatively, micro/nanopatterns spaced at large distances.
Key words
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Whitesides, G.M., Ostuni, E., Takayama, S., Jiang, X.Y., Ingber, D.E. (2001) Soft lithography in biology and biochemistry. Annu Rev Biomed Eng. 3, 335–373.
Kane, R. S., Takayama, S., Ostuni, E., Ingber, D. E., Whitesides, G. M. (1999) Patterning proteins and cells using soft lithography. Biomaterials 20, 2363–2376.
Xia, Y., Whitesides, G.M. (1998) Soft lithography. Angew Chem Int Ed 37, 550–575.
Hui, C. Y., Jagota, A., Lin, Y. Y., Kramer, E. J. (2002) Constraints on microcontact printing imposed by stamp deformation. Langmuir 18, 1394–1407.
Lauer, L., Klein C., Offenhäusser, A. (2001) Spot compliant neuronal networks by structure optimized micro-contact printing. Biomaterials 22, 1925–1932.
Delamarche, E., Schmid, H., Biebuyck, H. A., Michel, B. (1997) Stability of molded polydimethylsiloxane microstructures. Adv. Mater. 9, 741–746.
Bessueille, F., Pla-Roca, M., Mills, C. A., Martinez, E., Samitier, J., Errachid A. (2005) Submerged microcontact printing (SμCP): an unconventional printing technique of thiols using high aspect ratio, elastomeric stamps. Langmuir 21, 12060–12063.
Renault, J.P., Bernard, A., Bietsch, A., Michel, B., Bosshard, H.R., Delamarche, E., Kreiter, M., Hecht, B., Wild, U.P. (2003) Fabricating arrays of single protein molecules on glass using microcontact printing, J. Phys. Chem. B 107, 703–711.
Hong-Wei, L., Beinn, V. O., Muir, G. F., Huck, W. T. S. (2003) Nanocontact printing: a route to sub-50-nm-scale chemical and biological patterning. Langmuir 19, 1963–65.
Pla-Roca, M., Fernandez, J. G., Mills, C. A., Martínez, E., Samitier J. (2007) Micro/nanopatterning of proteins via contact printing using high aspect ratio PMMA stamps and nanoimprint apparatus. Langmuir 23, 8614–8618.
Acknowledgments
E.M. is grateful to the Spanish Ministry of Science and Education for the provision of grants through the I3 system. We acknowledge financial support from the Spanish Ministerio de Ciencia e Innovación through the project entitled “Regenerative Stem Cell Therapies for Heart Failure” and from the Science Support Program of the Fundación Marcelino Botín.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Martínez, E., Pla-Roca, M., Samitier, J. (2012). Micro/Nanopatterning of Proteins Using a Nanoimprint-Based Contact Printing Technique. In: Navarro, M., Planell, J. (eds) Nanotechnology in Regenerative Medicine. Methods in Molecular Biology, vol 811. Humana Press. https://doi.org/10.1007/978-1-61779-388-2_5
Download citation
DOI: https://doi.org/10.1007/978-1-61779-388-2_5
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-387-5
Online ISBN: 978-1-61779-388-2
eBook Packages: Springer Protocols