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Modification and Labeling of AAV Vector Particles

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Book cover Adeno-Associated Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 807))

Abstract

Adeno-associated virus (AAV) has become a versatile vector platform. In recent years, powerful ­techniques for the generation of tropism-modified vectors (rAAV-targeting vectors) and for investigation of virus–cell interaction were developed. The following chapter describes strategies for insertion of peptide ligands into the viral capsid and the subsequent characterization of capsid mutants, for producing mosaic capsids and for labeling the viral capsid chemically or genetically.

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Author information

Authors and Affiliations

  1. Department of Internal Medicine, University of Cologne, Cologne, Germany

    Hildegard Büning & Michael Hallek

  2. Center for Molecular Medicine Cologne (ZMMK), University of Cologne, Cologne, Germany

    Hildegard Büning & Michael Hallek

  3. Gene Therapy Center, The Research Institute at Nationwide Children’s Hospital, Columbus, OH, USA

    Chelsea M. Bolyard & Jeffrey S. Bartlett

  4. Integrated Biomedical Sciences Graduate Program, School of Medicine, The Ohio State University, Columbus, OH, USA

    Chelsea M. Bolyard

  5. Department of Pediatrics, and Department of Molecular Virology, Immunology, and Medical Genetics, The Ohio State University Columbus, Columbus, OH, USA

    Jeffrey S. Bartlett

Authors
  1. Hildegard Büning
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  2. Chelsea M. Bolyard
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  3. Michael Hallek
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  4. Jeffrey S. Bartlett
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Corresponding author

Correspondence to Hildegard Büning .

Editor information

Editors and Affiliations

  1. Center of Excellence for Regenerative, Health Biotechnology (CERHB), University of Florida, Innovation Drive 13706, Alachua, 32615, Florida, USA

    Richard O. Snyder

  2. INSERM U649, Labo. Thérapie Génique, CHU Hôtel Dieu, bd. Jean Monnet 30, Nantes CX 1, 44035, France

    Philippe Moullier

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Büning, H., Bolyard, C.M., Hallek, M., Bartlett, J.S. (2012). Modification and Labeling of AAV Vector Particles. In: Snyder, R., Moullier, P. (eds) Adeno-Associated Virus. Methods in Molecular Biology, vol 807. Humana Press. https://doi.org/10.1007/978-1-61779-370-7_12

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  • DOI: https://doi.org/10.1007/978-1-61779-370-7_12

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-369-1

  • Online ISBN: 978-1-61779-370-7

  • eBook Packages: Springer Protocols

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