Abstract
Alterations in DNA methylation patterns are implicated in playing a major role in the development of cancer, thus highlighting the need to continually develop new technologies to analyze epigenetic marks. Methylated-CpG Island Recovery Assay (MIRA), based on the high affinity of the MBD2b/MDB3L1 complex for double-stranded methylated DNA, allows for the recovery of methylated DNA without the use of bisulfite conversion or antibody recognition. MIRA is capable of detecting low-density methylation of a single methylated CpG nucleotide. This technique can be used in conjunction with microarrays or next-generation sequencing to analyze recovered methylated DNA on a genome-wide scale.
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Acknowledgments
This work was supported in part by a grant from the National Cancer Institute (RO1 CA129415). J.T.D. was supported by an NCI Cancer Prevention and Control Training Program (R25CA047888).
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Mitchell, N., DeAngelis, J.T., Tollefsbol, T.O. (2011). Methylated-CpG Island Recovery Assay. In: Tollefsbol, T. (eds) Epigenetics Protocols. Methods in Molecular Biology, vol 791. Humana Press. https://doi.org/10.1007/978-1-61779-316-5_10
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DOI: https://doi.org/10.1007/978-1-61779-316-5_10
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