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Neuropeptides pp 287–294Cite as

Neuropeptidases

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 789))

Abstract

The control of neuropeptide function is partially accomplished by aminopeptidases (neuropeptidases), which are the most abundant proteolytic enzymes in brain. Their analysis represents an important and quick tool to reflect the functional status of their endogenous substrates. Here, we describe an improved fluorometric method for the determination of neuropeptidase activities based on the fluorescence produced by β-naphthylamine when released from the artificial substrates aminoacyl-β-naphthylamides (arylamides) under the hydrolytic action of these enzymes.

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References

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Acknowledgments

This work was supported by Junta de Andalucía through PAI CVI-221 (Peptides and Peptidases) and BIO-221 (Neuroendocrinology and Nutrition). In Memorium of Francisco Alba.

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Authors and Affiliations

  1. Unit of Physiology, Department of Health Sciences, University of Jaén, Jaén, Spain

    Manuel Ramírez, Isabel Prieto, Inmaculada Banegas & Ana B. Segarra

  2. Department of Biochemistry and Molecular Biology III and Immunology, University of Granada Medical School, Granada, Spain

    Francisco Alba

Authors
  1. Manuel Ramírez
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  2. Isabel Prieto
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  3. Inmaculada Banegas
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  4. Ana B. Segarra
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  5. Francisco Alba
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Corresponding author

Correspondence to Manuel Ramírez .

Editor information

Editors and Affiliations

  1. , Dipt. di Morfofisiologia Veterinaria, Università degli Studi di Torino, Via Leonardo da Vinci 44, Grugliasco, 10095, Torino, Italy

    Adalberto Merighi

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Ramírez, M., Prieto, I., Banegas, I., Segarra, A.B., Alba, F. (2011). Neuropeptidases. In: Merighi, A. (eds) Neuropeptides. Methods in Molecular Biology, vol 789. Humana Press. https://doi.org/10.1007/978-1-61779-310-3_18

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  • DOI: https://doi.org/10.1007/978-1-61779-310-3_18

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-309-7

  • Online ISBN: 978-1-61779-310-3

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