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Laser Capture Microdissection

Volume 755 of the series Methods in Molecular Biology pp 395-403

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Laser-Capture Microdissection for Factor VIII-Expressing Endothelial Cells in Cancer Tissues

  • Tomoatsu KanekoAffiliated withCariology, Operative Dentistry and Endodontics, Niigata University Graduate School of Medical and Dental Sciences Email author 
  • , Takashi OkijiAffiliated withCariology, Operative Dentistry and Endodontics, Niigata University Graduate School of Medical and Dental Sciences
  • , Reika KanekoAffiliated withApplied Molecular Medicine, Niigata University Graduate School of Medical and Dental Sciences
  • , Hideaki SudaAffiliated withPulp Biology and Endodontics, Department of Restorative Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University
  • , Jacques E. NörAffiliated withCariology, Restorative Sciences, and Endodontics, School of Dentistry, University of MichiganDepartment of Biomedical Engineering, College of Engineering, University of MichiganComprehensive Cancer Center, University of Michigan

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Abstract

The immunostaining based Laser-capture microdissection (LCM) method called immune-LCM allows us to quantify the mRNA. Immune-LCM has recently been introduced to enhance identification of cells carrying a particular protein from frozen tissue samples. We have recently performed the immune-LCM of formaldehyde-fixated, paraffin-embedded tissues immunostained with a monoclonal antibody Factor VIII. This method could be useful for quantitative gene expression analysis in blood vessels from tumors of patients that have been treated with antiangiogenic drugs, allowing for validation of the effect of drug on the expected targets. Such capability might be exceedingly useful for the evaluation of the bioactivity of new drugs. This method is also useful to compare gene expression patterns in tumor cells versus endothelial cells during tumor progression or tumor angiogenesis.

Key words

Laser-capture microdissection Endothelial cells Gene expression Immune-LC FFPE tissues