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DNA Nanotechnology pp 105–113Cite as

Preparation and Atomic Force Microscopy of Quadruplex DNA

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 749))

Abstract

The purpose of this chapter is to provide detailed instructions for the preparation and atomic force microscopy (AFM) imaging of linear chains of quadruplex DNA (a.k.a. “G-wire DNA”). Successful self-assembly of long chain quadruplex DNA requires pure concentrated guanine-rich oligonucleotide sequence (GROs) and monovalent cations in a growth buffer. AFM imaging of individual G-wire DNA strands requires many carefully monitored steps, including substrate preparation, G-wire concentration, adsorption onto substrate, rinsing, drying, appropriate selection/use of imaging probes, and dry atmosphere imaging conditions. Detailed step-wise instructions are provided.

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Author information

Authors and Affiliations

  1. Department of Chemistry and Physics, University of New England, Biddeford, ME, USA

    James Vesenka

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  1. James Vesenka
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Correspondence to James Vesenka .

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Editors and Affiliations

  1. Dipto. Biochimica, Università Bologna, Via Irnerio 48, Bologna, 40126, Italy

    Giampaolo Zuccheri

  2. Dipto. Biochimica, Università Bologna, Via Irnerio 48, Bologna, 40126, Italy

    Bruno Samorì

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Vesenka, J. (2011). Preparation and Atomic Force Microscopy of Quadruplex DNA. In: Zuccheri, G., Samorì, B. (eds) DNA Nanotechnology. Methods in Molecular Biology, vol 749. Humana Press. https://doi.org/10.1007/978-1-61779-142-0_8

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  • DOI: https://doi.org/10.1007/978-1-61779-142-0_8

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-141-3

  • Online ISBN: 978-1-61779-142-0

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