Abstract
This chapter describes the LC-MS/MS methods for the determination of antibiotics residues in food matrices. The types of antibiotics include β-lactam antibiotics, sulfonamides, tetracyclines, fluoroquinolones, nitrofurans, and chloramphenicol (CAP). The food matrices are mainly from animal origin, such as animal tissues, fishes (marine products), eggs, milk, honey, and so on. The methods and procedures are covered, including three parts: (1) Liquid chromatographic conditions, (2) mass spectrometer conditions, including ionization source, analyzer, and acquisition, and (3) extraction and clean-up methods. In each case, the standard operating procedures (SOPs) for analysis are given with sensitivity, linearity, precision, and recovery. Some criteria of maximum residue limits (MRLs) from the legislation are listed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Cristina Blasco, Carmen Maria Torres, Yolanda Pico. Progress in analysis of residual antibacterials in food. Trends in Analytical Chemistry, 26( No. 9, 2007):895–913
A.A.M. Stolker, U.A.Th. Brinkman. Analytical strategies for residue analysis of veterinary drugs and growth-promoting agents in food-producing animals—a review. Journal of Chromatography A, 1067 (2005): 15–53
Urairat Koesukwiwat, Siripastr Jayanta, Natchanun Leepipatpiboon. Validation of a liquid chromatography–mass spectrometry multi-residue method for the simultaneous determination of sulfonamides, tetracyclines, and pyrimethamine in milk. Journal of Chromatography A, 1140 (2007): 147–156
Tara A. McGlincheya, Paul A. Raftera, Fiona Reganb, Gillian P. McMahonb. A review of analytical methods for the determination of aminoglycoside and macrolide residues in food matrices. analytica chimica acta 624 ( 2008 ): 1–15
Sara Bogialli , Giuseppe D’Ascenzo, Antonio Di Corcia, Aldo Lagana`, Simone Nicolardi. A simple and rapid assay based on hot water extraction and liquid chromatography–tandem mass spectrometry for monitoring quinolone residues in bovine milk. Food Chemistry 108 (2008): 354–360
M.J. Gonz´alez, de la Huebra, U. Vincent. Analysis of macrolide antibiotics by liquid chromatography. Journal of Pharmaceutical and Biomedical Analysis 39 (2005): 376–398
Sandra Pe´rez, Damia` Barcelo´. Applications of LC-MS to quantitation and evaluation of the environmental fate of chiral drugs and their metabolites. Trends in Analytical Chemistry, 27 ( No. 10, 2008): 836∼846
Linling Wang, Hai Yang, Chunwei Zhang, Yulin Mo, Xiaohua Lu. Determination of oxytetracycline, tetracycline and chloramphenicol antibiotics in animal feeds using subcritical water extraction and high performance liquid chromatography. analytica chimica acta 619 (2008 ): 54–58
Oscar Nu´n˜ ez, Encarnacio´n Moyano, Maria Teresa Galceran. LC–MS/MS analysis of organic toxics in food. Trends in Analytical Chemistry, 24 ( No. 7, 2005): 683∼703
Irene van den Broeka,∗, RolfW. Sparidansa, Jan H.M. Schellensa, Jos H. Beijnena. Quantitative bioanalysis of peptides by liquid chromatography coupled to (tandem) mass spectrometry. Journal of Chromatography B, 872 (2008): 1–22
M. Silvia Dı´az-Cruz, Damia` Barcelo. Recent advances in LC-MS residue analysis of veterinary medicines in the terrestrial environment. Trends in Analytical Chemistry, 26( No. 6, 2007):637∼646
M. Espinosa Bosch, A.J. Ruiz Sánchez, F. Sánchez Rojas, C. Bosch Ojeda. Recent developments in analytical determination of furosemide. Journal of Pharmaceutical and Biomedical Analysis 48 (2008): 519–532
A. Conneely, A. Nugent and M. O’ Keeffe. Use of solid phase extraction for the isolation and clean-up of a derivatised furazolidone metabolite from animal tissues Analyst 127 (6, 2002): 705–709
The Maximum Residue Limits of Residues of Veterinary Drugs in Animal Derived Food, issued No. 235 bulletin (2002) by Ministry of Agriculture, People’s Republic of China
European Commission, Commission Decision (2002/657/EC) of 12 August 2002
Acknowledgment
The authors are very grateful to Qin Feng and the other staff of Shanghai Institute for Food and Drug Control for using the LC QqQ mass spectrometers and supplying chromatograms.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2011 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Chen, GL., Fang, YY. (2011). The LC-MS/MS Methods for the Determination of Specific Antibiotics Residues in Food Matrices. In: Zweigenbaum, J. (eds) Mass Spectrometry in Food Safety. Methods in Molecular Biology, vol 747. Humana Press. https://doi.org/10.1007/978-1-61779-136-9_13
Download citation
DOI: https://doi.org/10.1007/978-1-61779-136-9_13
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-61779-135-2
Online ISBN: 978-1-61779-136-9
eBook Packages: Springer Protocols