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Nanoscale Biocatalysis

Volume 743 of the series Methods in Molecular Biology pp 77-93

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Kinesin I ATPase Manipulates Biohybrids Formed from Tubulin and Carbon Nanotubes

  • Cerasela Zoica DinuAffiliated withDepartment of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Center for Biotechnology and Interdisciplinary StudiesDepartment of Chemical Engineering, West Virginia University Email author 
  • , Shyam Sundhar BaleAffiliated withDepartment of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Center for Biotechnology and Interdisciplinary Studies
  • , Jonathan S. DordickAffiliated withDepartment of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Center for Biotechnology and Interdisciplinary Studies

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Abstract

This chapter describes a method for the formation of novel protein–nanotube hybrid conjugates. Specifically, we took advantage of the self-assembly and self-recognition properties of tubulin cytoskeletal protein immobilized onto carbon nanotubes to form nanotube-based biohybrids. Further biohybrid hierarchical integration in assemblies enabled molecular-level manipulation on engineered surfaces, as demonstrated with biocatalyst kinesin 1 ATPase molecular motor. The method presented herein can be extended for the preparation of biocatalyst-based or protein-based assemblies to be used as sensors or biological templates for nanofabrication.

Key words

Tubulin carbon nanotube self-assembly self-recognition biohybrid kinesin-based manipulation