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Cystic Fibrosis pp 143–171Cite as

Methods to Classify Bacterial Pathogens in Cystic Fibrosis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 742))

Abstract

Many bacteria can be detected in CF sputum, pathogenic and commensal. Modified Koch’s criteria for identification of established and emerging CF pathogens are therefore described. Methods are described to isolate bacteria and to detect bacterial biofilms in sputum or lung tissue from CF patients by means of conventional culturing and staining techniques and by the PNA FISH technique. Additionally, the confocal scanning laser microscopy technique is described for studying biofilms in vitro in a flow cell system. The recA-gene PCR and the RFLP-based identification methods are described for identification of isolates from the Burkholderia complex to the species level. DNA typing by PFGE, which can be used for any bacterial pathogen, is described as it is employed for Pseudomonas aeruginosa. A commercially available ELISA method is described for measuring IgG antibodies against P. aeruginosa in CF patients.

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Authors and Affiliations

  1. Department of Clinical Microbiology, University of Copenhagen, 2100, Copenhagen, Denmark

    Thomas Bjarnsholt, Xiaohui Chen Nielsen, Ulla Johansen, Lena Nørgaard & Niels Høiby

  2. Danish CF Centre, Rigshospitalet, University of Copenhagen, 2100, Copenhagen, Denmark

    Thomas Bjarnsholt & Niels Høiby

  3. Institute of International Health, Immunology and Microbiology, University of Copenhagen, 2100, Copenhagen, Denmark

    Niels Høiby

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  1. Thomas Bjarnsholt
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  2. Xiaohui Chen Nielsen
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  3. Ulla Johansen
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  4. Lena Nørgaard
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  5. Niels Høiby
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Correspondence to Niels Høiby .

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Editors and Affiliations

  1. Faculty of Sciences, Centre for Biodiversity & Functional, University of Lisboa, Campo Grande, Lisboa, 1749-016, Portugal

    Margarida D. Amaral

  2. , Department of Physiology, University of Regensburg, Universitätsstr. 31, Regensburg, 93053, Germany

    Karl Kunzelmann

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Bjarnsholt, T., Nielsen, X.C., Johansen, U., Nørgaard, L., Høiby, N. (2011). Methods to Classify Bacterial Pathogens in Cystic Fibrosis. In: Amaral, M., Kunzelmann, K. (eds) Cystic Fibrosis. Methods in Molecular Biology, vol 742. Humana Press. https://doi.org/10.1007/978-1-61779-120-8_9

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  • DOI: https://doi.org/10.1007/978-1-61779-120-8_9

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