Abstract
Escherichia coli O157:H7, a major cause of food-borne illness, produces Shiga toxins (Stxs) that block protein synthesis by inactivating the ribosome. In this chapter, we describe a simple cell-based fluorescent assay to detect Stxs and inhibitors of toxin activity. The assay can also be used to detect other plant and bacterial toxins that arrest protein synthesis.
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Acknowledgments
The authors thank high school student Srikar Reddy and undergraduate Jessica Johnston for assistance with generating the dose-response curves for ricin, diphtheria toxin, and exotoxin A.
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Massey, S., Quiñones, B., Teter, K. (2011). A Cell-Based Fluorescent Assay to Detect the Activity of Shiga Toxin and Other Toxins that Inhibit Protein Synthesis. In: Holst, O. (eds) Microbial Toxins. Methods in Molecular Biology, vol 739. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-102-4_5
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DOI: https://doi.org/10.1007/978-1-61779-102-4_5
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-61779-101-7
Online ISBN: 978-1-61779-102-4
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