Abstract
Fumonisin mycotoxins are common contaminants in many grains, often at very low levels. Maize is particularly problematic as one of the organisms that commonly produce fumonisins, the fungus Fusarium verticillioides, often exists as an endophyte of maize. Fumonisin is a potent inhibitor of the enzyme ceramide synthase, and this inhibition results in the accumulation of a variety of upstream compounds, most notably, the sphingoid bases sphingosine, sphinganine, 1-deoxysphinganine and, in plants, phytosphingosine. Fumonisin exposure results in a wide variety of species, sex, and strain-specific responses. This method provides a relatively fast means of extracting fumonisins, sphingoid bases, and sphingoid base 1-phosphates from tissues and cells, as well as the subsequent analyses and quantification of these compounds using liquid chromatography/tandem mass spectrometry.
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Cook RJ (1981) Water relations in the biology of Fusarium. In: Nelson PE, Toussoun TA, Cook RJ (eds) Fusarium: diseases, biology, and taxonomy. The Pennsylvania State University Press, University Park, PA
Kommedahl T, Windels CE (1981) Root-, stalk-, and ear-infecting Fusarium species on corn in the USA. In: Nelson PE, Toussoun TA, Cook RJ (eds) Fusarium: diseases, biology, and taxonomy. The Pennsylvania State University Press, University Park, PA
EHC 219 (2000) Environmental health criteria 219: fumonisin B1. In: Marasas WHO, Miller JD, Riley RT, Visconti A (eds) International Programme on Chemical Safety. United Nations Environmental Programme, The International Labour Organization, and the World Health Organization, Geneva, Switzerland, pp 1–150
Marasas WFO, Kellerman TS, Gelderbloom WCA, Coetzer JAW, Theil PG, Van der Lugt JJ (1988) Leukoencephalomalacia in a horse induced by fumonisin B1 isolated from Fusarium moniliforme. Onderstepoort J Vet Res 55:197–203
Ross PF, Nelson PE, Richard JL, Osweiler GD, Rice LG, Plattner RD et al (1990) Production of fumonisins by Fusarium moniliforme and Fusarium proliferatum isolates associated with equine leukoencephalomalacia and a pulmonary edema syndrome in swine. Appl Environ Microbiol 56:3225–3226
IARC (2002) Some traditional medicines, some mycotoxins, naphthalene and styrene (IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, vol 82). IARC Press, Lyon, France, pp 275–366
Marasas WFO, Riley RT, Hendricks KA, Stevens VL, Sadler TW, Gelineau-van WJ et al (2004) Fumonisins disrupt sphingolipid metabolism, folate transport, and neural tube development in embryo culture and in vivo: a potential risk factor for human neural tube defects among populations consuming fumonisin-contaminated maize. J Nutr 134:711–716
Williams LD, Glenn AE, Zimeri AM, Bacon CW, Smith MA, Riley RT (2007) Fumonisin disruption of ceramide biosynthesis in maize roots and the effects on plant development and Fusarium verticillioides-induced seedling disease. J Agric Food Chem 55:2937–2946
Desjardins AE, Plattner RD, Nelsen TC, Leslie JF (1995) Genetic analysis of fumonisin production and virulence of Gibberella fujikuroi mating population A (Fusarium moniliforme) on maize (Zea mays) seedlings. Appl Environ Microbiol 61:79–86
Rheeder JP, Marasas WFO, Vismer HF (2002) Production of fumonisin analogs by Fusarium species. Appl Environ Microbiol 68:2101–2105
Bartok T, Szecsi A, Szekeres A, Mesterhazy A, Bartok M (2006) Detection of new fumonisin mycotoxins and fumonisin-like compounds by reversed-phase high-performance liquid chromatography/electrospray ionization ion trap mass spectrometry. Rapid Commun Mass Spectrom 20:2447–2462
Wang E, Norred WP, Bacon CW, Riley RT, Merrill AH Jr (1991) Inhibition of sphingolipid biosynthesis by fumonisins. Implications for diseases associated with Fusarium moniliforme. J Biol Chem 266:14486–14490
Riley RT, Enongene E, Voss KA, Norred WP, Meredith FI, Sharma RP et al (2001) Sphingolipid perturbations as mechanisms for fumonisin carcinogenesis. Environ Health Perspect 109:301–308
Abbas HK, Tanaka T, Duke SO, Porter JK, Wray EM, Hodges L et al (1994) Fumonisin- and AAL-toxin-induced disruption of sphingolipid metabolism with accumulation of free sphingoid bases. Plant Physiol 106:1085–1093
Merrill AH, Jr SMC, Wang E, Voss KA, Riley RT (2001) Sphingolipid metabolism: roles in signal transduction and disruption by Fumonisins. Environ Health Perspect 109:283–289
Maceyka M, Milstien S, Spiegel S (2009) Sphingosine-1-phosphate: the Swiss army knife of sphingolipid signaling. J Lipid Res 50:S272–S276
Ng CK-Y, Hetherington AM (2001) Sphingolipid-mediated signalling in plants. Ann Bot 88:957–965
Worrall D, Ng CK-Y, Hetherington AM (2003) Sphingolipids, new players in plant signaling. Trends Plant Sci 8:317–320
Lynch DV, Dunn TM (2004) An introduction to plant sphingolipids and a review of recent advances in understanding their metabolism and function. New Phytol 61:677–702
Coursol S, Stunff HE, Lynch DV, Gilroy S, Assman SM, Spiegel S (2005) Arabidopsis sphingosine kinase and the effects of phytosphingosine-1-phosphate on stomatal aperture. Plant Physiol 137:724–737
Shi L, Bielawski J, Mu J, Dong H, Teng C, Zhang J et al (2007) Involvement of sphingoid bases in mediating reactive oxygen intermediate production and programmed cell death in Arabidopsis. Cell Res 17:1030–1140
Glenn AE, Zitomer NC, Zimeri AM, Williams LD, Riley RT, Proctor RH (2008) Transformation-mediated complementation of a FUM gene cluster deletion in Fusarium verticillioides restores both fumonisin production and pathogenicity on maize seedlings. Mol Plant Microbe Interact 21:87–97
Zitomer NC, Glenn AE, Bacon CW, Riley RT (2008) A single extraction method for the analysis by liquid chromatography/tandem mass spectrometry of fumonisins and biomarkers of disrupted sphingolipid metabolism in tissues of maize seedlings. Anal Bioanal Chem 391:2257–2263
Riley RT, Voss KA (2006) Differential sensitivity of rat kidney and liver to fumonisin toxicity: organ-specific differences in toxin accumulation and sphingoid base metabolism. Toxicol Sci 92:335–345
Enongene EN, Sharma RP, Bhandari N, Miller JD, Meredith FI, Voss KA et al (2002) Persistence and reversibility of the elevation in free sphingoid bases induced by fumonisin inhibition of ceramide synthase. Toxicol Sci 67:173–181
Zitomer NC, Mitchell T, Voss KA, Bondy GS, Pruett ST, Garnier-Amblard EC et al (2009) Ceramide synthase inhibition by fumonisin B1 causes accumulation of 1-deoxysphinganine: a novel category of bioactive 1-deoxysphingoid bases and 1-deoxyhydroceramides biosynthesized by mammalian cell lines and animals. J Biol Chem 284:4786–4795
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Zitomer, N.C., Riley, R.T. (2011). Extraction and Analysis of Fumonisins and Compounds Indicative of Fumonisin Exposure in Plant and Mammalian Tissues and Cultured Cells. In: Holst, O. (eds) Microbial Toxins. Methods in Molecular Biology, vol 739. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-102-4_15
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DOI: https://doi.org/10.1007/978-1-61779-102-4_15
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