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Biomedical Nanotechnology

Volume 726 of the series Methods in Molecular Biology pp 207-221

Date:

Viruses as Nanomaterials for Drug Delivery

  • Dustin Lockney
  • , Stefan FranzenAffiliated withDepartment of Chemistry, North Carolina State University Email author 
  • , Steven Lommel

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Abstract

Virus delivery vectors are one among the many nanomaterials that are being developed as drug delivery materials. This chapter focuses on methods utilizing plant virus nanoparticles (PVNs) synthesized from the Red clover necrotic mosaic virus (RCNMV). A successful vector must be able to effectively carry and subsequently deliver a drug cargo to a specific target. In the case of the PVNs, we describe two types of ways cargo can be loaded within these structures: encapsidation and infusion. Several targeting approaches have been used for PVNs based on bioconjugate chemistry. Herein, examples of such approaches will be given that have been used for RCNMV as well as for other PVNs in the literature. Further, we describe characterization of PVNs, in vitro cell studies that can be used to test the efficacy of a targeting vector, and potential routes for animal administration.

Key words

Bioconjugation Capsid Drug delivery Encapsidation Infusion Nanomaterial Peptides Plant virus nanoparticle Targeting