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Regulatory T Cells pp 105–118Cite as

Genetic Tools for Analysis of FoxP3+ Regulatory T Cells In Vivo

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 707))

Abstract

The discovery of Foxp3 as a reliable marker for murine regulatory T cells has led to an explosion in the development of genetic tools for investigating the biology of regulatory T cells. More than 25 Foxp3-based mouse strains have been published with a variety of characteristics. The effects of Foxp3 expression can be analyzed using null, hypomorphic, conditional, altered control, and over-expression strains. Reporter strains are available to efficiently isolate Foxp3+ cells, with various reporter designs in terms of construct (fusion, replacement, and bicistronic positioning), and reporter system (GFP, YFP, RFP, Luciferase, Thy1.1). Multifunction strain fusion, replacement, and bicistronic positionings add functional proteins under the control of the Foxp3 promoter allowing induced apoptosis or lineage-specific Cre recombinase activity. In this chapter, we discuss the uses of the cornucopia of genetic tools, in isolation and in combination, for research on Foxp3+ regulatory T cells.

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Authors and Affiliations

  1. VIB Autoimmune Genetics Laboratory, K.U. Leuven, Leuven, Belgium

    Nadia M. Jeremiah & Adrian Liston

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  1. Nadia M. Jeremiah
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  2. Adrian Liston
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Correspondence to Adrian Liston .

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Editors and Affiliations

  1. , Division of Immunoregulation, MRC National Institute for Medical Resea, The Ridgeway, Mill Hill, London, NW7 1AA, United Kingdom

    George Kassiotis

  2. , VIB Autoimmune Genetics Laboratory, Katholieke Universiteit Leuven, Herestraat 49, Leuven, 3000, Belgium

    Adrian Liston

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Jeremiah, N.M., Liston, A. (2011). Genetic Tools for Analysis of FoxP3+ Regulatory T Cells In Vivo. In: Kassiotis, G., Liston, A. (eds) Regulatory T Cells. Methods in Molecular Biology, vol 707. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61737-979-6_8

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  • DOI: https://doi.org/10.1007/978-1-61737-979-6_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61737-978-9

  • Online ISBN: 978-1-61737-979-6

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