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Antigen-Specific Induction of Regulatory T Cells In Vivo and In Vitro

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Regulatory T Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 707))

Abstract

The peripheral induction of Foxp3-expressing regulatory T cells outside the thymus is required in order to maintain local homeostasis in distinct microenvironments such as the gut. Extrathymic induction of Treg may also be exploited to prevent unwanted immune responses. Here, we discuss the methodology allowing for the stable de novo generation of Tregs specific for foreign antigens in peripheral lymphoid tissue via subimmunogenic peptide delivery using either peptide contained in fusion antibodies directed against the DEC205 endocytotic receptor on steady-state dendritic cells or the implantation of peptide-delivering osmotic mini-pumps. Furthermore, we also address methods in order to achieve TGFβ-dependent Treg conversion in vitro, thereby mainly focusing on the role of retinoic acid (RA) to enhance TGFβ-dependent conversion into Tregs.

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Acknowledgments

These studies were supported by NIH grant NIH-AI-53102 to Harald von Boehmer. Carolin Daniel was supported by a Leopoldina research fellowship (BMBF-LPD 9901/8-184) and by LOEWE (LiFF) program of the Federal State of Hessen, Germany.

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Authors and Affiliations

  1. Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, USA

    Carolin Daniel

  2. Department of Biology, Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA, USA

    Hidde Ploegh

  3. Laboratory of Molecular Biology, Medical Research Council, Cambridge, USA

    Harald von Boehmer

Authors
  1. Carolin Daniel
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  2. Hidde Ploegh
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  3. Harald von Boehmer
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Corresponding author

Correspondence to Harald von Boehmer .

Editor information

Editors and Affiliations

  1. , Division of Immunoregulation, MRC National Institute for Medical Resea, The Ridgeway, Mill Hill, London, NW7 1AA, United Kingdom

    George Kassiotis

  2. , VIB Autoimmune Genetics Laboratory, Katholieke Universiteit Leuven, Herestraat 49, Leuven, 3000, Belgium

    Adrian Liston

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Daniel, C., Ploegh, H., von Boehmer, H. (2011). Antigen-Specific Induction of Regulatory T Cells In Vivo and In Vitro. In: Kassiotis, G., Liston, A. (eds) Regulatory T Cells. Methods in Molecular Biology, vol 707. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61737-979-6_11

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  • DOI: https://doi.org/10.1007/978-1-61737-979-6_11

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61737-978-9

  • Online ISBN: 978-1-61737-979-6

  • eBook Packages: Springer Protocols

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