Abstract
Transcript profiling has improved our understanding of quorum-sensing gene regulation on a global scale. Here, we describe methods for high-quality sample preparation for use in DNA microarray and real-time PCR experiments.
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Acknowledgements
Parts of the cDNA synthesis procedure are based on a protocol by Matt Wolfgang, University of North Carolina. Thanks to Joon-Hee Lee, University of Pusan, South Korea, for his suggestions on real-time PCR.
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Schuster, M. (2011). Global Expression Analysis of Quorum-Sensing Controlled Genes. In: Rumbaugh, K. (eds) Quorum Sensing. Methods in Molecular Biology, vol 692. Humana Press. https://doi.org/10.1007/978-1-60761-971-0_13
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DOI: https://doi.org/10.1007/978-1-60761-971-0_13
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