Abstract
Current models for systemic sclerosis have many limitations for mimicking human disease. We have recently described a novel model based on chronic stimulation of skin by poly(I:C), a form of double-stranded RNA known to stimulate innate immune responses. This model shows inflammation and extracellular matrix deposition, and upregulated expression of genes associated with fibrosis and vascular injury. We detail here the methodology for this model, utilizing osmotic pumps to deliver innate immune stimuli to subcutaneous tissue. Subcutaneous osmotic pumps provide a flexible system for studying innate immunity in the skin as well as the affects of other ligands on skin inflammation and fibrosis.
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References
http://www.alzet.com/products/guide_to_use/pump_selection.html
Kawai T, Akira S (2008) Toll-like receptor and RIG-I-like receptor signaling. Ann NY Acad Sci 1143:1–20
Farina G, York M, Collins C, Lafyatis R (2010) dsRNA activation of endothelin-1 and markers of vascular activation in endothelial cells and fibroblasts. Ann Rheum Dis 70:544–550
Farina GA, York MR, Di Marzio M, Collins CA, Meller S, Homey B, Rifkin IR, Marshak-Rothstein A, Radstake TR, Lafyatis R (2010) Poly(I:C) drives type I IFN- and TGFbeta-mediated inflammation and dermal fibrosis simulating altered gene expression in systemic sclerosis. J Invest Dermatol 130:2583–2593
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© 2012 Springer Science+Business Media New York
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Dimarzio, M., Farina, G., Lafyatis, R. (2012). Modeling Innate Immunity in Murine Skin: Utilization of Subcutaneous Osmotic Pumps for Inflammatory and Fibrotic Skin Disease. In: Perl, A. (eds) Autoimmunity. Methods in Molecular Biology, vol 900. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-720-4_15
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DOI: https://doi.org/10.1007/978-1-60761-720-4_15
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-719-8
Online ISBN: 978-1-60761-720-4
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