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Synthesis, Characterization, and Functionalization of Gold Nanoparticles for Cancer Imaging

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Cancer Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 624))

Abstract

This chapter describes the methodology by which mAb-F19-conjugated gold nanoparticles were prepared and used to label human pancreatic adenocarcinoma. Specifically, gold nanoparticles were coated with dithiol bearing hetero-bifunctional PEG (polyethylene glycol), and cancer-specific mAb F19 was attached by means of NHS-EDC coupling chemistry taking advantage of a carboxylic acid group on the heterobifunctional PEG. These conjugates were completely stable and were characterized by a variety of methods, including UV–Vis absorbance spectrometry, darkfield microscopy, DLS (dynamic light scattering), TEM (transmission electron microscopy), SEC (size-exclusion chromatography), and confocal microscopy. Nanoparticle bioconjugates were used to label sections of healthy and cancerous human pancreatic tissue. Labeled tissue sections were examined by darkfield microscopy and indicate that these nanoparticle bioconjugates may selectively bind to cancerous tissue and provide a means of optical contrast.

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Author information

Authors and Affiliations

  1. Department of Chemical and Biological Engineering, University of Maine, Orono, ME, USA

    Gary A. Craig & Michael D. Mason

  2. Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, USA

    Peter J. Allen

Authors
  1. Gary A. Craig
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  2. Peter J. Allen
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  3. Michael D. Mason
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Editor information

Editors and Affiliations

  1. Dept. Surgery, University of Florida, Gainesville, USA

    Stephen R. Grobmyer

  2. ERC - Engineering Research Center, University of Florida, Gainesville, USA

    Brij M. Moudgil

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Craig, G.A., Allen, P.J., Mason, M.D. (2010). Synthesis, Characterization, and Functionalization of Gold Nanoparticles for Cancer Imaging. In: Grobmyer, S., Moudgil, B. (eds) Cancer Nanotechnology. Methods in Molecular Biology, vol 624. Humana Press. https://doi.org/10.1007/978-1-60761-609-2_12

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  • DOI: https://doi.org/10.1007/978-1-60761-609-2_12

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-608-5

  • Online ISBN: 978-1-60761-609-2

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