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Glycoprotein Characterization

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Book cover Functional Glycomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 600))

Abstract

Increasing numbers of studies are reporting the modification of prokaryotic proteins with novel glycans. These proteins are often associated with virulence factors of medically important pathogens. Herein, we describe the steps required to characterize prokaryotic glycoproteins by mass spectrometry, using flagellin isolated from Clostridium botulinum strain Langeland as an example. Both “top-down” and “bottom-up” approaches will be described for characterizing the purified glycoprotein at the whole protein and peptide levels. The preliminary steps toward structural characterization of novel prokaryotic glycans by mass spectrometry and NMR are also described.

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Acknowledgments

We thank Dr Catherine Paul for isolation of C. botulinum flagellin proteins and James Mullen for assistance with preliminary mass spectrometry characterizations of flagellin. We also thank Kelly Fulton for assistance with final manuscript preparations.

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Authors and Affiliations

  1. Institute for Biological Sciences, National Research Council Canada, Ottawa, Ontario, Canada

    Susan M. Twine, Luc Tessier & John F. Kelly

Authors
  1. Susan M. Twine
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  2. Luc Tessier
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  3. John F. Kelly
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Editor information

Editors and Affiliations

  1. Inst. Biological Sciences, National Research Council Canada, Sussex Drive 100, Ottawa, K1A 0R6, Canada

    Jianjun Li

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© 2010 Humana Press

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Twine, S.M., Tessier, L., Kelly, J.F. (2010). Glycoprotein Characterization. In: Li, J. (eds) Functional Glycomics. Methods in Molecular Biology, vol 600. Humana Press. https://doi.org/10.1007/978-1-60761-454-8_8

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  • DOI: https://doi.org/10.1007/978-1-60761-454-8_8

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-453-1

  • Online ISBN: 978-1-60761-454-8

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