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Bioremediation pp 103–116Cite as

Combination of Fluorescence In Situ Hybridization with Staining Techniques for Cell Viability and Accumulation of PHA and polyP in Microorganisms in Complex Microbial Systems

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 599))

Abstract

Fluorescence in situ hybridization (FISH) can be combined with a number of staining techniques to reveal the relationships between the microorganisms and their function in complex microbial systems with a single-cell resolution. In this chapter, we have focused on staining methods for intracellular storage compounds (polyhydroxyalkanoates, polyphosphate) and a measure for cell viability, reduction of the tetrazolium-based redox stain CTC. These protocols are optimized for the study of microorganisms in waste-water treatment (activated sludge and biofilms), but they may also be used with minor modifications in many other ecosystems.

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Authors and Affiliations

  1. Department of Biotechnology, Chemistry and Environmental Engineering, Aalborg University, Aalborg, Denmark

    Jeppe Lund Nielsen, Caroline Kragelund & Per Halkjær Nielsen

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  1. Jeppe Lund Nielsen
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  2. Caroline Kragelund
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  3. Per Halkjær Nielsen
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Editors and Affiliations

  1. School of Applied Sciences, Northumbria University, Ellison Place, Newcastle upon Tyne, NE1 8ST, United Kingdom

    Stephen P. Cummings

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© 2010 Humana Press

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Nielsen, J.L., Kragelund, C., Nielsen, P.H. (2010). Combination of Fluorescence In Situ Hybridization with Staining Techniques for Cell Viability and Accumulation of PHA and polyP in Microorganisms in Complex Microbial Systems. In: Cummings, S. (eds) Bioremediation. Methods in Molecular Biology, vol 599. Humana Press. https://doi.org/10.1007/978-1-60761-439-5_7

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  • DOI: https://doi.org/10.1007/978-1-60761-439-5_7

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  • Print ISBN: 978-1-60761-440-1

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