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Inflammation and Cancer pp 209–232Cite as

Inhibiting Proinflammatory NF-κB Signaling Using Cell-Penetrating NEMO Binding Domain Peptides

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 512))

Summary

Nuclear factor kappa B (NF-κB) is an inducible transcription factor that regulates the expression of many genes involved in normal immune and inflammatory responses. NF-κB activation is normally a rapid and transient response to pro-inflammatory stimuli however dysregulated constitutively active NF-κB signaling leads to chronic inflammation and provides a cell survival signal in many types of cancer. NF-κB signaling is therefore an important target for the development of novel anti-inflammatory or anti-cancer drugs. We previously identified and characterized a cell-permeable peptide that blocks NF-κB signaling by disrupting the critical upstream IκB kinase (IKK) complex. We describe in this chapter three separate methods to determine the effects of this NEMO-binding domain (NBD) peptide on pro-inflammatory NF-κB signaling in response to tumor necrosis factor (TNF).

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Acknowledgments

Work in the authors' laboratory was supported by RO1 HL080612

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Authors and Affiliations

  1. Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Lisa A. Madge & Michael J. May

Authors
  1. Lisa A. Madge
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  2. Michael J. May
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Editor information

Editors and Affiliations

  1. National Cancer Institute at Frederick, SAIC-Frederick, Inc., Frederick, MD, USA

    Serguei V. Kozlov

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Madge, L.A., May, M.J. (2009). Inhibiting Proinflammatory NF-κB Signaling Using Cell-Penetrating NEMO Binding Domain Peptides. In: Kozlov, S.V. (eds) Inflammation and Cancer. Methods in Molecular Biology™, vol 512. Humana Press. https://doi.org/10.1007/978-1-60327-530-9_11

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  • DOI: https://doi.org/10.1007/978-1-60327-530-9_11

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-529-3

  • Online ISBN: 978-1-60327-530-9

  • eBook Packages: Springer Protocols

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