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Affinity Chromatography of Pyrogens

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Book cover Affinity Chromatography

Part of the book series: Methods in Molecular Biology ((MIMB,volume 147))

Abstract

Exogenous pyrogens originating in the cell wall of Gram-negative bacteria have the strongest pyrogenicity (1), and for them synonyms such as endotoxins, lipopolysaccharides (LPS), and O-antigens are used. A number of attempts using affinity chromatographic approaches have been made to remove pyrogens. These are based on the specific adsorption of pyrogens, and therefore might be useful for removal of pyrogens from various solutions containing biologically active substances. One such approach is based upon the use of affinity adsorbents that contain polymyxin B as a ligand (2,3). However, polymyxin B is strongly toxic to the central nervous system and the kidneys (4) and therefore is not suitable as a ligand for removal of pyrogens from a solution, which will be used for intravenous injection. Among affinity adsorbents, immobilized histidine might be the most favorable adsorbent for removal of pyrogens because of safety, stability, and cost (5–7). Therefore, in this chapter, we describe the preparation, characteristics, and applications of immobilized histidine.

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References

  1. Nowotny A. (1969) Molecular aspects of endotoxic reaction. Bacteriol. Rev. 33, 72–98.

    PubMed  CAS  Google Scholar 

  2. Issektz A. C. (1983) Removal of gram-negative endotoxin from solutions by affinity chromatography. Immunol. Methods 61, 275–281.

    Article  Google Scholar 

  3. Kodama M., Hanasawa K., and Tani T. (1990) New therapeutic method against septic shock-removal of endotoxin using extracorporeal circulation, in Advances in Experimental Medicine and Biology, Vol. 256 (Endotoxin) (Friedman H., Klein T. W., Nakano M., and Nowotny A., eds.) Plenum New York, pp. 653–664.

    Google Scholar 

  4. Srinivasa B. R. and Ramachandran L. K. (1979) The polymyxins. J. Sci. Ind. Res. 38, 695–709.

    CAS  Google Scholar 

  5. Minobe S., Watanabe T., Sato T., and Tosa T. (1988) Characteristics and applications of adsorbents for pyrogen removal. Biotechnol. Appl. Biochem. 10, 143–153.

    PubMed  CAS  Google Scholar 

  6. Matsumae H., Minobe S., Kindan K., Watanabe T., Sato T., and Tosa T. (1990) Specific removal of endotoxin from protein solutions by immobilized histidine. Biotechnol. Appl. Biochem. 12, 129–140.

    PubMed  CAS  Google Scholar 

  7. Tosa T., Sato T., Watanabe T., and Minobe S. (1993) Affinity chromatographic removal of pyrogens, in Molecular Interactions in Bioseparations (Ngo T. T., ed.), Plenum, New York, pp. 323–332.

    Google Scholar 

  8. Minobe S., Watanabe T., Sato T., Tosa T., and Chibata I. (1982) Preparation of adsorbents for pyrogen adsorption. J. Chromatogr. 248, 401–408.

    Article  CAS  Google Scholar 

  9. Minobe S., Nawata M., Watanabe T., Sato T., and Tosa T. (1991) Specific assay for endotoxin using immobilized histidine and Limulus amebocyte lysate. Anal. Biochem. 198, 292–297.

    Article  PubMed  CAS  Google Scholar 

  10. Nawata M., Minobe S., Hase M., Watanabe T., Sato T., and Tosa T. (1992) Specific assay for endotoxin using immobilized histidine, Limulus amoebocyte lysate and a chromogenic substrate. J. Chromatogr. 597, 415–424.

    Article  PubMed  CAS  Google Scholar 

  11. Minobe S., Nawata M., Shigemori N., and Watanabe T. (1994) Assay of endotoxin in human plasma using immobilized histidine, Limulus amoebocyte lysate and Chromogenic substrate. Eur. J. Clin. Chem. Clin. Biochem. 32, 797–803.

    PubMed  CAS  Google Scholar 

  12. Shiomi S., Kuroki T., Ueda T., Takeda T., Nishiguchi S., Nakajima S., Kobayashi K., Yamagami S., Watanabe T., and Minobe S. (1994) Use of immobilized histidine in assay for endotoxin in patients with liver disease. J. Gastroenterol. 29, 751–755.

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Pharmaceutical Development Research Laboratory, Tanabe Seiyaku Co. Ltd., Osaka, Japan

    Satoshi Minobe, Takeji Shibatani & Tetsuya Tosa

  2. Department of Quality Control, Osaka Plant, Tanabe Seiyaku Co., Ltd., Osaka, Japan

    Satoshi Minobe

Authors
  1. Satoshi Minobe
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  2. Takeji Shibatani
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  3. Tetsuya Tosa
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Editor information

Editors and Affiliations

  1. Department of Biopharmaceuticals, Hoffmann-La Roche Inc., Nutley, NJ

    Pascal Bailon , George K. Ehrlich , Wen-Jian Fung  & Wolfgang Berthold , ,  & 

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© 2000 Humana Press Inc.

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Minobe, S., Shibatani, T., Tosa, T. (2000). Affinity Chromatography of Pyrogens. In: Bailon, P., Ehrlich, G.K., Fung, WJ., Berthold, W. (eds) Affinity Chromatography. Methods in Molecular Biology, vol 147. Humana Press. https://doi.org/10.1007/978-1-60327-261-2_15

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  • DOI: https://doi.org/10.1007/978-1-60327-261-2_15

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-694-9

  • Online ISBN: 978-1-60327-261-2

  • eBook Packages: Springer Protocols

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