Skip to main content
SpringerLink
Log in
Book cover

Characterization of Nanoparticles Intended for Drug Delivery pp 83–91Cite as

Biological Tissue and Cell Culture Specimen Preparation for TEM Nanoparticle Characterization

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 697))

Abstract

This chapter outlines the procedures for ex vivo TEM preparation of nanoparticle-containing tissue or cell culture samples using an epoxy resin embedding method. The purpose of this procedure is to preserve the structure of tissue in a hardened epoxy block with minimal disruption of cellular structures, to aid in the meaningful analysis of in vivo or cell culture experiments. The process begins with hydrated tissue and ends with tissue that is virtually water-free and preserved in a static state within a plastic resin matrix. The resin mixture permeates the dehydrated tissue, making the sample firm enough to cut. Procedures are also given for fixing nanoparticle-containing cell culture samples.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   79.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   99.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   139.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Hayat, M.A. (1970) Principles and techniques of electron microscopy. Van Nostrand Reinhold Company, New York.

    Google Scholar 

  2. Bozzola, J.J. and Russell, L.D. (1999) Electron microscopy: Principles and techniques for biologists. Jones and Bartlett Publishers, New York.

    Google Scholar 

Download references

Acknowledgment

This project has been funded in whole or in part by federal funds from the National Cancer Institute, National Institutes of Health, under contract N01-CO-12400. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does the mention of trade names, commercial products, or organizations imply endorsement by the US Government.

Author information

Authors and Affiliations

  1. Electron Microscopy Laboratory, Advanced Technology Program, SAIC – Frederick, Inc., National Cancer Institute at Frederick, Frederick, MD, USA

    Kunio Nagashima

  2. Nanotechnology Characterization Laboratory, Advanced Technology Program, SAIC – Frederick, Inc., National Cancer Institute at Frederick, Frederick, MD, USA

    Jiwen Zheng, David Parmiter & Anil K. Patri

Authors
  1. Kunio Nagashima
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jiwen Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. David Parmiter
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Anil K. Patri
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Anil K. Patri .

Editor information

Editors and Affiliations

  1. SAIC-Frederick, Inc., Nanotechnology Characterization Lab, National Cancer Institute at Frederick, Boyles Street 1050, Frederick, 21702, Maryland, USA

    Scott E. McNeil

Rights and permissions

Reprints and permissions

Copyright information

© 2011 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Nagashima, K., Zheng, J., Parmiter, D., Patri, A.K. (2011). Biological Tissue and Cell Culture Specimen Preparation for TEM Nanoparticle Characterization. In: McNeil, S. (eds) Characterization of Nanoparticles Intended for Drug Delivery. Methods in Molecular Biology, vol 697. Humana Press. https://doi.org/10.1007/978-1-60327-198-1_8

Download citation

  • DOI: https://doi.org/10.1007/978-1-60327-198-1_8

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-197-4

  • Online ISBN: 978-1-60327-198-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation