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Characterization of Nanoparticles Intended for Drug Delivery pp 145–153Cite as

Quantitation of Nanoparticles in Serum Matrix by Capillary Electrophoresis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 697))

Abstract

Sensitive and fast analytical techniques are needed to determine the concentration of nanoparticles in biological samples (e.g., blood and tissues) for biodistribution and toxicity studies. This chapter describes a method for the use of capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) for the quantitation of fullerene nanoparticles in human serum matrix. Data on the fullerene-based nanoparticle carboxyfullerene (C3 fullerene) in human serum is presented as an example.

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Acknowledgments

This project has been funded in whole or in part by federal funds from the National Cancer Institute, National Institutes of Health, under contract N01-CO-12400. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does the mention of trade names, commercial products, or organizations imply endorsement by the US Government.

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Authors and Affiliations

  1. Laboratory of Proteomics and Analytical Technologies, Advanced Technology Program, SAIC – Frederick, Inc., National Cancer Institute at Frederick, Frederick, MD, USA

    King C. Chan, Timothy D. Veenstra & Haleem J. Issaq

Authors
  1. King C. Chan
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  2. Timothy D. Veenstra
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  3. Haleem J. Issaq
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Editor information

Editors and Affiliations

  1. SAIC-Frederick, Inc., Nanotechnology Characterization Lab, National Cancer Institute at Frederick, Boyles Street 1050, Frederick, 21702, Maryland, USA

    Scott E. McNeil

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Chan, K.C., Veenstra, T.D., Issaq, H.J. (2011). Quantitation of Nanoparticles in Serum Matrix by Capillary Electrophoresis. In: McNeil, S. (eds) Characterization of Nanoparticles Intended for Drug Delivery. Methods in Molecular Biology, vol 697. Humana Press. https://doi.org/10.1007/978-1-60327-198-1_15

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  • DOI: https://doi.org/10.1007/978-1-60327-198-1_15

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60327-197-4

  • Online ISBN: 978-1-60327-198-1

  • eBook Packages: Springer Protocols

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