Summary
Immunonaffinity chromatography is a powerful technique for rapid purification of proteins. In a single-step purification, it is possible to purify proteins for testing in model systems and for conducting enzyme kinetic studies. Because the immunoaffinity-purified proteins are typically >90–95% pure, depending on the starting material, interference from remaining contaminants is rare. This method describes an immunoaffinity chromatography technique for purifying proteins from over-expression in mammalian cell culture. The immobilization of the monoclonal antibody or polyclonal antiserum is presented. Conditions for purifying up to milligram quantities of protein are given, including a representative chromatogram.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Gallant, S.R., Koppaka, V., Zecherle, N. (2008). Immunoaffinity Chromatography. In: Zachariou, M. (eds) Affinity Chromatography. Methods in Molecular Biology™, vol 421. Humana Press. https://doi.org/10.1007/978-1-59745-582-4_4
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DOI: https://doi.org/10.1007/978-1-59745-582-4_4
Publisher Name: Humana Press
Print ISBN: 978-1-58829-659-7
Online ISBN: 978-1-59745-582-4
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