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Gene Function Analysis pp 257–290Cite as

A Bacterial/Yeast Merged Two-Hybrid System

Protocol for Yeast Screening With Single or Parallel Baits

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 408))

Abstract

The yeast two-hybrid system is a useful tool for identifying new protein-protein interactions, and for the dissection of previously identified interactions. An important issue in protein-interaction studies is frequently that of determining whether a protein associates specifically with one protein or domain of interest, or has a more promiscuous interaction profile. To help address this issue, the authors have created a new two-hybrid system, which can be used either in bacteria or in yeast to counterscreen against “decoy” baits in parallel with a primary screen, hence improving the power and specificity of the method. Protocols of this system for use in yeast are provided; a companion article, Serebriiski et al., describes alternative use of this system in bacteria.

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Author information

Authors and Affiliations

  1. Fox Chase Cancer Center, Philadelphia, PA

    Nadezhda Y. Tikhmyanova, Eugene A. Izumchenko, Ilya G. Serebriiskii & Erica A. Golemis

  2. Drexel University School of Medicine, Philadelphia, PA

    Nadezhda Y. Tikhmyanova

  3. Ben Gurion University, Beer Sheva, Israel

    Eugene A. Izumchenko

Authors
  1. Nadezhda Y. Tikhmyanova
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  2. Eugene A. Izumchenko
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  3. Ilya G. Serebriiskii
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  4. Erica A. Golemis
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Editor information

Editors and Affiliations

  1. The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Baltimore, MD

    Michael F. Ochs

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© 2007 Humana Press Inc.

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Tikhmyanova, N.Y., Izumchenko, E.A., Serebriiskii, I.G., Golemis, E.A. (2007). A Bacterial/Yeast Merged Two-Hybrid System. In: Ochs, M.F. (eds) Gene Function Analysis. Methods in Molecular Biology™, vol 408. Humana Press. https://doi.org/10.1007/978-1-59745-547-3_15

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  • DOI: https://doi.org/10.1007/978-1-59745-547-3_15

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-734-1

  • Online ISBN: 978-1-59745-547-3

  • eBook Packages: Springer Protocols

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