Abstract
The use of protein arrays and their importance in proteomic applications continues to be at the forefront of scientific discovery and innovative technology development. To date, array-based approaches have proven to be a powerful tool for protein expression profiling, novel biomarker discovery, and the examination of protein, DNA, and small molecule interactions. Our laboratory has developed several approaches for characterizing protein-protein interactions using protein microarrays for a variety of different biological applications. Here we describe the identification of protein-protein interactions using a microarray format.
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References
Albala, J. S. (2001) Array-based proteomics: the latest chip challenge. Exp. Rev. Mol. Diag. 1(2), 145–152.
Cahill, D. J. (2001) Protein and antibody arrays and their medical applications. J. Immunol. Methods 250(1–2), 81–91.
Poetz, O., Schwenk, J. M., Kramer, S., et al. (2005) Protein microarrays: catching the proteome. Mech. Ageing Dev. 126(1), 161–170.
MacBeath, G. and Schreiber, S. L. (2000) Printing proteins as microarrays for high-throughput function determination. Science 289(5485), 1760–1763.
Zhu, H., Klemic, J. F., Chang, S., et al. (2000) Analysis of yeast protein kinases using protein chips. Nat. Genet. 26(3), 283–229.
Ramachandran, N., Hainsworth, E., Bhullar, B., et al. (2004) Self-assembling protein microarrays. Science 305(5680), 86–90.
Haab, B. B., Dunham, M. J., and Brown, P. D. (2001) Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions. Genome Biol. 2(2), research 0004.1–0004.13.
Coleman, M. A., Miller, K. A., Beernink, P. T., et al. (2003) Identification of chromatin-related protein interactions using protein microarrays. Proteomics 3(11), 2101–2107.
Coleman, M. A., Lao, V. H., and Segelke, B. W. (2004) High-throughput, fluorescence based screening for soluble protein expression. J. Proteome Res. 3(5), 1024–1032.
Albala, J. S., Franke, K., McConnell, I. R., et al. (2000) From genes to proteins: high-throughput expression and purification of the human proteome. J. Cell Biochem. 80(2), 187–191.
Segelke, B. W., Schafer, J., Coleman, M. A., et al. (2004) Laboratory scale structural genomics. J. Struct. Funct. Genomics 5(12), 147–157.
Lennon, G., Auffray, C., Polymeropoulos, M., et al. (1996) The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression. Genomics 33, 151–152.
Gilbert, M., Edwards, T. C., and Albala, J. S. (2004) Protein expression arrays for proteomics. Methods Mol. Biol. 264, 15–23.
Murthy, T. V., Wu, W., Qiu, Q. Q., et al. (2004) Bacterial cell-free system for high-throughput protein expression and a comparative analysis of Escherichia coli cell-free and whole cell expression systems. Protein Expr. Purif. 36(2), 217–225.
Gilbert, M. and Albala, J. S. (2002) Accelerating code to function: sizing up the protein production line. Curr. Opin. Chem. Biol. 6(1), 102–105.
Waldo, G., Standish, B. M., Berendzen, J., and Terwilliger, T. C. (1999) Rapid protein-folding assay using green fluorescent protein. Nat. Biotechnol. 17(7), 691–695.
Rao, R. S., Visuri, S. R., McBride, M. T., Albala, J. S., Matthews, D. L., and Coleman, M. A. (2004) Comparison of multiplexed techniques for detection of bacterial and viral proteins. J. Proteome Res. 3(4), 736–742.
Camarero, J. A., Kwon, Y., and Coleman, M. A. (2004) Chemoselective attachment of biologically active proteins to surfaces by expressed protein ligation and its application for “protein chip” fabrication. J. Am. Chem. Soc. 126(45), 14,730–14,731.
Zhou, H., Bouwman, K., Schotanus, M., et al. (2004) Two-color, rolling-circle amplification on antibody microarrays for sensitive, multiplexed serum-protein measurements. Genome Biol. 5(4), R28.
Kononen, J., Bubendorf, L., Kallioniemi, A., et al. (1998)Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat. Med. 4(7), 844–847.
Huang, J. X., Mehrens, D., Wiese, R., et al. (2001) High-throughput genomic and proteomic analysis using microarray technology. Clin. Chem. 47(10), 1912–1916.
Pawlak, M., Schick, E., Bopp, M. A., et al. (2002) Zeptosens’ protein microarrays: a novel high performance microarray platform for low abundance protein analysis. Proteomics 2(4), 383–393.
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© 2007 Humana Press Inc., Totowa, NJ
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Coleman, M.A., Beernink, P.T., Camarero, J.A., Albala, J.S. (2007). Applications of Functional Protein Microarrays. In: Floriano, P.N. (eds) Microchip-Based Assay Systems. Methods in Molecular Biology™, vol 385. Humana Press. https://doi.org/10.1007/978-1-59745-426-1_9
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DOI: https://doi.org/10.1007/978-1-59745-426-1_9
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