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Microchip Methods in Diagnostics pp 169–179Cite as

Detection of Enteropathogenic Escherichia coli by Microchip Capillary Electrophoresis

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 509))

Summary

There is always a need to detect the presence of microorganisms, either as contaminants in food and pharmaceutical industries or bioindicators for disease diagnosis. Hence, it is important to develop efficient, rapid, and simple methods to detect microorganisms. Traditional culturing method is unsatisfactory due to its long incubation time. Molecular methods, although capable of providing a high degree of specificity, are not always useful in providing quick tests of presence or absence of microorganisms. Microchip elec-trophoresis has been recently employed to address problems associated with the detection of microorganisms due to its high versatility, selectivity, sensitivity, and short analysis times. In this work, the potential of PDMS-based microchip electrophoresis in the identification and characterization of microorganism was evaluated. Enteropathogenic E. coli (EPEC) was selected as the model microorganism. To obtain repeat-able separations, sample pretreatment was found to be essential. Microchip electrophoresis with laser-induced fluorescence detection could potentially revolutionize certain aspects of microbiology involving diagnosis, profiling of pathogens, environmental analysis, and many others areas of study.

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Author information

Authors and Affiliations

  1. Department of Chemistry, National University of Singapore, Singapore, Republic of Singapore

    Wai S. Law & Sam F.Y. Li

  2. Department of Pathology & Laboratory Medicine, University of Pennsylvania Medical Center, Philadelphia, PA, USA

    Larry J. Kricka

Authors
  1. Wai S. Law
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  2. Sam F.Y. Li
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  3. Larry J. Kricka
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Editor information

Editors and Affiliations

  1. Helmholtz Centre for Infection Research, Braunschweig, Germany

    Ursula Bilitewski

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© 2009 Springer Science+Business Media, LLC

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Law, W.S., Li, S.F., Kricka, L.J. (2009). Detection of Enteropathogenic Escherichia coli by Microchip Capillary Electrophoresis. In: Bilitewski, U. (eds) Microchip Methods in Diagnostics. Methods in Molecular Biology™, vol 509. Humana Press. https://doi.org/10.1007/978-1-59745-372-1_12

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  • DOI: https://doi.org/10.1007/978-1-59745-372-1_12

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-955-0

  • Online ISBN: 978-1-59745-372-1

  • eBook Packages: Springer Protocols

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