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DNA Microarrays: An Introduction to the Technology

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Microchip Methods in Diagnostics

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 509))

Summary

DNA microarrays allow the comprehensive genetic analysis of an organism or a sample. They are based on probes, which are immobilized in an ordered two-dimensional pattern on substrates, such as nylon membranes or glass slides. Probes are either spotted cDNAs or oligonucleotides and are designed to be specific for an organism, a gene, a genetic variant (mutation or polymorphism), or intergenic regions. Thus, they can be used for example for genotyping, expression analysis, or studies of protein–DNA interactions, and in the biomedical field they allow the detection of pathogens, antibiotic resistances, gene mutations and polymorphisms, and pathogenic states and can guide therapy. Microarrays, which cover the whole genome of an organism, are as well available as those which are focussed on genes related to a certain diagnostic application.

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Authors and Affiliations

  1. Helmholtz Centre for Infection Research, Braunschweig, Germany

    Ursula Bilitewski

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  1. Ursula Bilitewski
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Editors and Affiliations

  1. Helmholtz Centre for Infection Research, Braunschweig, Germany

    Ursula Bilitewski

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© 2009 Springer Science+Business Media, LLC

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Bilitewski, U. (2009). DNA Microarrays: An Introduction to the Technology. In: Bilitewski, U. (eds) Microchip Methods in Diagnostics. Methods in Molecular Biology™, vol 509. Humana Press. https://doi.org/10.1007/978-1-59745-372-1_1

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  • DOI: https://doi.org/10.1007/978-1-59745-372-1_1

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-955-0

  • Online ISBN: 978-1-59745-372-1

  • eBook Packages: Springer Protocols

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