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The Protein Protocols Handbook pp 25–34Cite as

Ultrafast Protein Determinations Using Microwave Enhancement

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Abstract

In this chapter, we describe modifications of existing protein assays that take advantage of microwave irradiation to reduce assay incubation times from the standard 15–60 min down to just seconds (1).

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References

  1. Akins, R. E. and Tuan, R. S. (1992) Measurement of protein in 20 seconds using a microwave BCA assay. Biotechniques 12, 496–499.

    CAS  PubMed  Google Scholar 

  2. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275.

    CAS  PubMed  Google Scholar 

  3. Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Proven-zano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., and Klenk, D. C. (1985) Measurement of protein using bicinchoninic acid. Analyt. Biochem. 150, 76–85.

    Article  CAS  PubMed  Google Scholar 

  4. Kok, L. P. and Boon, M. E. (1992) Microwave Cookbook for Microscopists. Coulomb, Leyden, Leiden, Netherlands.

    Google Scholar 

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Acknowledgements

This work was supported in part by funds from NASA-SBRA and AHA 9406244S (REA), as well as NIH HD 15822 and NIH HD 29937 (RST).

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Authors and Affiliations

  1. Tissue Engineering and Regenerative Medicine Research, Nemours Biomedical Research, A.I. duPont Hospital for Children, Wilmington, DE, USA

    Robert E. Akins

  2. Department of Orthopaedic Surgery, Department of Biochemistry and Molecular Pharmacology, Cell and Tissue Engineering Graduate Program, Thomas Jefferson University, Philadelphia, PA, USA

    Rocky S. Tuan

Authors
  1. Robert E. Akins
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  2. Rocky S. Tuan
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Editor information

Editors and Affiliations

  1. School of Life Sciences, University of Hertfordshire, Hatfield, Hertfordshire, UK

    John M. Walker

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Akins, R.E., Tuan, R.S. (2009). Ultrafast Protein Determinations Using Microwave Enhancement. In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-198-7_5

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  • DOI: https://doi.org/10.1007/978-1-59745-198-7_5

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-474-6

  • Online ISBN: 978-1-59745-198-7

  • eBook Packages: Springer Protocols

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