Abstract
Shortly after penetration into the cell, HIV-1 must reverse transcribe its genome into a double-stranded DNA molecule and must gain access to the nucleus of nondividing cells for productive infection. There is limited knowledge of these early events in HIV-1 life cycle. We have developed methods, which allow the study of postpenetration events and in particular allow the isolation and purification of intracellular complexes mediating reverse transcription. Such complexes can be examined for their structure, function, protein composition and ability to access the nucleus.
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Acknowledgments
AF is a Wellcome Trust University Fellow. I thank Steve Goff, Masha Orlova, Eran Bacharach and Guanxia Gao for their help and advise for the development of these methods.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Fassati, A. (2008). Methods of Preparation and Analysis of Intracellular Reverse Transcription Complexes. In: Prasad, V.R., Kalpana, G.V. (eds) HIV Protocols. Methods In Molecular Biology™, vol 485. Humana Press. https://doi.org/10.1007/978-1-59745-170-3_8
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DOI: https://doi.org/10.1007/978-1-59745-170-3_8
Publisher Name: Humana Press
Print ISBN: 978-1-58829-859-1
Online ISBN: 978-1-59745-170-3
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