Abstract
Autoradtography ts the locahzed recording of a radtolabel wtthm a solid specimen The term does not apply to any single technique There are many ways of producing an image on a photographic emulsion The quality and accuracy of the image depends on many factors, mcluding the autoradiographic technique, the tsotope employed, and the photographic medium used to record the image The best results are obtained in the shortest possible time with the correct emulsion and exposure time There are constraints that can restrict resolution as well as sensitivity Some of these may be overcome by converting the emitted radiation to light, either by enclosing the autoradiography film between the radioacttve sample and a fluorescent-mtensifying screen or by mcorporating a scmtillator into the sample (fluorography).
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© 1998 Humana Press Inc , Totowa, NJ
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Harvey, B. (1998). Autoradiography and Fluorography. In: Rapley, R., Walker, J.M. (eds) Molecular Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-59259-642-3_11
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DOI: https://doi.org/10.1007/978-1-59259-642-3_11
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