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Molecular Biomethods Handbook pp 1–8Cite as

Extraction of Total RNA from Tissues and Cultured Cells

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Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

The rsolatron of intact, high quality, total cellular RNA is often the starting point for many molecular biologrcal procedures (Fig. 1) There are numerous general methods for the rsolation of total cellular RNA (1–9). There are also many specialrzed methods for the rsolatron of RNA from specific tissues (8–10), various cell types (11), and subcellular organelles (7,12,13) In addmon, a number of methods describe the srmultaneous rsolatton of RNA and DNA (14–17). Generally, the rationale for any tsolation procedure is to solubihze cellular components and simultaneously mactrvate mtracellular RNases while mamtaming brologrcally active RNA Therefore, the goal is to acquire purrfied cellular RNA in an intact form that can be a substrate for further mampulatrons, such as in vitro translation, RNase protectton, reverse transcrrptron, and Northern-blot analysis.

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Author information

Authors and Affiliations

  1. Department of Genetics, Hospital for Sick Children, Toronto, Canada

    Sandeep Raha, Mingfu Ling & Frank Merante

Authors
  1. Sandeep Raha
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  2. Mingfu Ling
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  3. Frank Merante
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Editor information

Editors and Affiliations

  1. University of Herfordshire, Hatfield, UK

    Ralph Rapley  & John M. Walker  & 

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© 1998 Humana Press Inc , Totowa, NJ

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Raha, S., Ling, M., Merante, F. (1998). Extraction of Total RNA from Tissues and Cultured Cells. In: Rapley, R., Walker, J.M. (eds) Molecular Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-59259-642-3_1

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  • DOI: https://doi.org/10.1007/978-1-59259-642-3_1

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-501-0

  • Online ISBN: 978-1-59259-642-3

  • eBook Packages: Springer Book Archive

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