Abstract
Density gradient ultracentrifugation has been widely used to fractionate animal, plant, and bacterial cells, viral particles, lysosomes, membranes, and mac-romolecules in a range of processes. Its application has been of particular significance in the commercial preparation of viruses for vaccine and immuno-therapy products in both batch and continuous-flow zonal modes. These methods have been traditionally used to purify influenza vaccines (1), but more recently have been used in devising new vaccine purifications such as Hepatitis B (2) and Rabies (3).
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References
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Desai, M.A., Merino, S.P. (2000). Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale Purification of Biomolecules. In: Desai, M.A. (eds) Downstream Processing of Proteins. Methods in Biotechnology, vol 9. Humana Press. https://doi.org/10.1007/978-1-59259-027-8_6
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DOI: https://doi.org/10.1007/978-1-59259-027-8_6
Publisher Name: Humana Press
Print ISBN: 978-0-89603-564-5
Online ISBN: 978-1-59259-027-8
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